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Qualitative and quantitative analysis of the major constituents in Jin-Mu-Gan-Mao tablet by high-performance liquid chromatography with diode-array detection and quadrupole time-of-flight tandem mass spectrometry
Huang M.1; Xu S.1; Xu W.1; Chen D.1; Chu K.1; Xu W.1; Peng J.1; Lu J.2
2014
Source PublicationJournal of Separation Science
ISSN16159314 16159306
Volume37Issue:23Pages:3497-3508
Abstract

Jin-Mu-Gan-Mao tablet is a well-known traditional Chinese medicinal preparation, which has been used to treat the common cold in China. In this study, a systematic method was established for the qualitative and quantitative analysis of the major constituents in Jin-Mu-Gan-Mao tablet. First, a method of high-performance liquid chromatography with diode-array detection and quadrupole time-of-flight mass spectrometry was developed for identification of the multi-constituents. Thirty-one compounds including ten phenolic acids, 18 flavonoids, and three iridoid glycosides were clearly identified by comparison with the reference standards, and 11 compounds were deduced by comparison with the literature data. Second, a new quantitative analysis method of Jin-Mu-Gan-Mao tablet was established by high-performance liquid chromatography with diode-array detection. Twelve compounds, either with high contents or strong bioactivities, were chosen as marker components. This analytical method was validated through intra-and interday precision, repeatability, and stability,with respective relative standard deviations less than 1.74, 2.54, 2.44, and 2.48%. The limits of detection and quantification were less than 0.327 and 0.881 μg/mL, respectively. The overall recoveries ranged from 97.04-102.76% (relative standard deviation ≥ 2.91%). Then this validated method was applied to determine ten batches of Jin-Mu-Gan-Mao tablet. The results indicated that these new approaches can be applicable for the qualitative and quantitative analysis of Jin-Mu-Gan-Mao tablet.

KeywordHigh-performance Liquid Chromatography Jin-mu-gan-mao Tablet Mass Spectrometry Quadrupole Time-of-flight Traditional Chinese Medicine
DOI10.1002/jssc.201400631
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000345829900012
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被引频次[WOS]:6   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.Fujian University of Traditional Chinese Medicine
2.Universidade de Macau
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Huang M.,Xu S.,Xu W.,et al. Qualitative and quantitative analysis of the major constituents in Jin-Mu-Gan-Mao tablet by high-performance liquid chromatography with diode-array detection and quadrupole time-of-flight tandem mass spectrometry[J]. Journal of Separation Science,2014,37(23):3497-3508.
APA Huang M..,Xu S..,Xu W..,Chen D..,Chu K..,...&Lu J..(2014).Qualitative and quantitative analysis of the major constituents in Jin-Mu-Gan-Mao tablet by high-performance liquid chromatography with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.Journal of Separation Science,37(23),3497-3508.
MLA Huang M.,et al."Qualitative and quantitative analysis of the major constituents in Jin-Mu-Gan-Mao tablet by high-performance liquid chromatography with diode-array detection and quadrupole time-of-flight tandem mass spectrometry".Journal of Separation Science 37.23(2014):3497-3508.
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