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Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D
Lu J.-J.1; Lu D.-Z.2; Chen Y.-F.2; Dong Y.-T.2; Zhang J.-R.2; Li T.1; Tang Z.-H.1; Yang Z.2
2015-09-01
Source PublicationChinese Journal of Natural Medicines
ISSN18755364
Volume13Issue:9Pages:673-679
Abstract

Platycodin D (PD), a triterpenoid saponin isolated from Platycodonis Radix, is a famous Chinese herbal medicine that has been shown to have anti-proliferative effects in several cancer cell lines. The aim of this study was to determine the changes in cellular proteins after the treatment of hepatocellular carcinoma HepG2 cells with PD using proteomics approaches. The cell viability was determined using the MTT assay. The proteome was analyzed by two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot analysis was used to confirm the expression of changed proteins. Our results showed that PD inhibited the proliferation of HepG2 cells in concentration- and time-dependent manners. Sixteen proteins were identified to be up-regulated in PD-treated HepG2 cells, including ATP5H, OXCT1, KRT9, CCDC40, ERP29, RCN1, ZNF175, HNRNPH1, HSP27, PA2G4, PHB, BANF1, TPM3, ECH1, LGALS1, and MYL6. Three proteins (i.e., RPS12, EMG1, and KRT1) decreased in HepG2 cells after treatment with PD. The changes in HSP27 and PHB were further confirmed by Western blotting. In conclusion, our results shed new lights on the mechanisms of action for the anti-cancer activity of PD.

Keyword2-d Dige Hepg2 Platycodin d Proteome
DOI10.1016/S1875-5364(15)30065-0
URLView the original
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Universidade de Macau
2.Zhejiang Chinese Medical University
Recommended Citation
GB/T 7714
Lu J.-J.,Lu D.-Z.,Chen Y.-F.,et al. Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D[J]. Chinese Journal of Natural Medicines,2015,13(9):673-679.
APA Lu J.-J..,Lu D.-Z..,Chen Y.-F..,Dong Y.-T..,Zhang J.-R..,...&Yang Z..(2015).Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D.Chinese Journal of Natural Medicines,13(9),673-679.
MLA Lu J.-J.,et al."Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D".Chinese Journal of Natural Medicines 13.9(2015):673-679.
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