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PK11195-chitosan-graft-polyethylenimine-modified SPION as a mitochondria-targeting gene carrier
Kim Y.-K.1; Zhang M.1; Lu J.-J.3; Xu F.1; Chen B.-A.2; Xing L.1; Jiang H.-L.1
2016-05-27
Source PublicationJournal of Drug Targeting
ISSN10292330 1061186X
Volume24Issue:5Pages:457-467
Abstract

Superparamagnetic iron oxide nanoparticle (SPION) holds great potential as a gene delivery system due to its unique properties, such as good biocompatibility and non-invasive targeting ability. In this study, we modified SPION with chitosan-graft-PEI (CHI-g-PEI) and PK11195, to fabricate a mitochondria-targeting gene carrier, PK-CP-SPION. PK-CP-SPION manifested prominent physicochemical properties for magnetic guided gene delivery, and it could effectively condense and protect DNA at proper weight ratios. The in vitro cytotoxicity of PK-CP-SPIONs was mild. Under an external magnetic field, the transfection efficiency of PK-CP-SPIONs was comparable to PEI 25 K with shorter transfection time. PK11195 facilitated the specific accumulation of PK-CP-SPIONs in mitochondria, leading to the leakage of cytochrome c, the dissipation of mitochondrial membrane potential and subsequently the activation of mitochondria apoptosis pathway. These results indicated that with further development, PK-CP-SPIONs could serve as a multifunctional nanoplatform for magnetic targeting gene delivery and mitochondria-targeting therapy, leading enhanced therapeutic effect towards tumor cells.

KeywordGene Therapy Magnetofection Mitochondria-targeting Non-viral Vector Superparamagnetic Iron Oxide Nanoparticles
DOI10.3109/1061186X.2015.1087527
URLView the original
Indexed BySCI
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000372756000010
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.China Pharmaceutical University
2.Southeast University
3.Universidade de Macau
Recommended Citation
GB/T 7714
Kim Y.-K.,Zhang M.,Lu J.-J.,et al. PK11195-chitosan-graft-polyethylenimine-modified SPION as a mitochondria-targeting gene carrier[J]. Journal of Drug Targeting,2016,24(5):457-467.
APA Kim Y.-K..,Zhang M..,Lu J.-J..,Xu F..,Chen B.-A..,...&Jiang H.-L..(2016).PK11195-chitosan-graft-polyethylenimine-modified SPION as a mitochondria-targeting gene carrier.Journal of Drug Targeting,24(5),457-467.
MLA Kim Y.-K.,et al."PK11195-chitosan-graft-polyethylenimine-modified SPION as a mitochondria-targeting gene carrier".Journal of Drug Targeting 24.5(2016):457-467.
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