UM  > 中華醫藥研究院
Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells
Bao H.1; Zhang L.-L.1; Liu Q.-Y.1; Feng L.2; Ye Y.2; Lu J.-J.1; Lin L.-G.1
2016-06-01
Source PublicationMolecules
ISSN14203049
Volume21Issue:6
Abstract

The chemical study on the seeds of Caesalpinia sappan led to the isolation of five new cassane diterpenoids, phanginins R-T (1-3) and caesalsappanins M and N (4 and 5), together with seven known compounds 6-12. Their structures were elucidated on the basis of NMR and HRESIMS analyses. The absolute configurations of compounds 1 and 4 were determined by the corresponding CD spectra. All the isolated compounds were tested for their cytotoxicity against ovarian cancer A2780 and HEY, gastric cancer AGS, and non-small cell lung cancer A549 cells. Compound 1 displayed significant toxicity against the four cell lines with the IC values of 9.9 ± 1.6 μM, 12.2 ± 6.5 μM, 5.3 ± 1.9 μM, and 12.3 ± 3.1 μM, respectively. Compound 1 induced G1 phase cell cycle arrest in A2780 cells. Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. What's more, compound 1 also promoted the expression of the tumor suppressor p53 protein. These findings indicate that cassane diterpenoids might have potential as anti-cancer agents, and further in vivo animal studies and structural modification investigation are needed.

KeywordCaesalpinia Sappan Cassane Diterpenoids Cytotoxicity Apoptosis P53
DOI10.3390/molecules21060791
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000378757600117
Fulltext Access
Citation statistics
Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLin L.-G.
Affiliation1.Universidade de Macau
2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Bao H.,Zhang L.-L.,Liu Q.-Y.,et al. Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells[J]. Molecules,2016,21(6).
APA Bao H..,Zhang L.-L..,Liu Q.-Y..,Feng L..,Ye Y..,...&Lin L.-G..(2016).Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells.Molecules,21(6).
MLA Bao H.,et al."Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells".Molecules 21.6(2016).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Bao H.]'s Articles
[Zhang L.-L.]'s Articles
[Liu Q.-Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Bao H.]'s Articles
[Zhang L.-L.]'s Articles
[Liu Q.-Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Bao H.]'s Articles
[Zhang L.-L.]'s Articles
[Liu Q.-Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.