UM  > 中華醫藥研究院
Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells
Bao H.1; Zhang L.-L.1; Liu Q.-Y.1; Feng L.2; Ye Y.2; Lu J.-J.1; Lin L.-G.1
2016-06-01
Source PublicationMolecules
ISSN14203049
Volume21Issue:6
Abstract

The chemical study on the seeds of Caesalpinia sappan led to the isolation of five new cassane diterpenoids, phanginins R-T (1-3) and caesalsappanins M and N (4 and 5), together with seven known compounds 6-12. Their structures were elucidated on the basis of NMR and HRESIMS analyses. The absolute configurations of compounds 1 and 4 were determined by the corresponding CD spectra. All the isolated compounds were tested for their cytotoxicity against ovarian cancer A2780 and HEY, gastric cancer AGS, and non-small cell lung cancer A549 cells. Compound 1 displayed significant toxicity against the four cell lines with the IC values of 9.9 ± 1.6 μM, 12.2 ± 6.5 μM, 5.3 ± 1.9 μM, and 12.3 ± 3.1 μM, respectively. Compound 1 induced G1 phase cell cycle arrest in A2780 cells. Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. What's more, compound 1 also promoted the expression of the tumor suppressor p53 protein. These findings indicate that cassane diterpenoids might have potential as anti-cancer agents, and further in vivo animal studies and structural modification investigation are needed.

KeywordCaesalpinia Sappan Cassane Diterpenoids Cytotoxicity Apoptosis P53
DOI10.3390/molecules21060791
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000378757600117
Fulltext Access
Citation statistics
Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLin L.-G.
Affiliation1.Universidade de Macau
2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Bao H.,Zhang L.-L.,Liu Q.-Y.,et al. Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells[J]. Molecules,2016,21(6).
APA Bao H..,Zhang L.-L..,Liu Q.-Y..,Feng L..,Ye Y..,...&Lin L.-G..(2016).Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells.Molecules,21(6).
MLA Bao H.,et al."Cytotoxic and pro-apoptotic effects of cassane diterpenoids from the seeds of Caesalpinia sappan in cancer cells".Molecules 21.6(2016).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Bao H.]'s Articles
[Zhang L.-L.]'s Articles
[Liu Q.-Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Bao H.]'s Articles
[Zhang L.-L.]'s Articles
[Liu Q.-Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Bao H.]'s Articles
[Zhang L.-L.]'s Articles
[Liu Q.-Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.