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Simultaneous determination of anthraquinones in Rhubarb by pressurized liquid extraction and capillary zone electrophoresis
Gong Y.X.1; Li S.P.1; Wang Y.T.1; Li P.1; Yang F.Q.1
2005-05-01
Source PublicationElectrophoresis
ISSN01730835
Volume26Issue:9Pages:1778-1782
AbstractRhubarb, a well-known Chinese herbal medicine, is also used in Europe and other places of the world. Anthraquinones derivatives are thought to be the major active components. A pressurized liquid extraction (PLE) and capillary zone electrophoresis (CZE) separation were developed for simultaneous determination of five anthraquinones including aloe-emodin, emodin, chrysophanol, physcion, and rhein in Rhubarb. The effects of the experimental variables on PLE and CZE have been optimized. The optimum conditions of PLE were: solvent, methanol; temperature, 140°C; particle size, 0.13-0.2 mm; static extraction time, 5 min; pressure, 1500 psi; and one extraction. The best separation of the five anthraquinones could be obtained using 50 mM borate buffer (pH 8.2) containing 25% isopropyl alcohol and 25% acetontrile as modifier, while the separation voltage was 25 kV and the temperature was at 20°C. The method developed is accurate, simple, and reproducible, and could be used for quality control of Rhubarb and its medical preparations. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
KeywordAnthraquinones Capillary zone electrophoresis Pressurized liquid extraction Quantitative determination Rhubarb
DOI10.1002/elps.200400001
URLView the original
Language英語
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Cited Times [WOS]:36   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Universidade de Macau
2.Anhui Medical University
Recommended Citation
GB/T 7714
Gong Y.X.,Li S.P.,Wang Y.T.,et al. Simultaneous determination of anthraquinones in Rhubarb by pressurized liquid extraction and capillary zone electrophoresis[J]. Electrophoresis,2005,26(9):1778-1782.
APA Gong Y.X.,Li S.P.,Wang Y.T.,Li P.,&Yang F.Q..(2005).Simultaneous determination of anthraquinones in Rhubarb by pressurized liquid extraction and capillary zone electrophoresis.Electrophoresis,26(9),1778-1782.
MLA Gong Y.X.,et al."Simultaneous determination of anthraquinones in Rhubarb by pressurized liquid extraction and capillary zone electrophoresis".Electrophoresis 26.9(2005):1778-1782.
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