UM
Simultaneous determination of seven phenolic acids in three Salvia species by capillary zone electrophoresis with β-cyclodextrin as modifier
Cao J.2; We J.2; Tian K.1; Su H.2; Wan J.2; Li P.2
2014
Source PublicationJournal of Separation Science
ISSN16159314 16159306
Volume37Issue:24Pages:3738-3744
AbstractA capillary zone electrophoresis method was developed for the simultaneous determination of seven phenolic acids, including protocatechuic aldehyde (1), salvianolic acid C (2), rosmarinic acid (3), salvianolic acid A (4), danshensu (5), salvianolic acid B (6), and protocatechuic acid (7), in Danshen and related medicinal plants. A running buffer composed of 20 mM sodium tetraborate adjusted to pH 9.0, and containing 12 mM β-cyclodextrin as modifier. Baseline separation was achieved within 17 min running at the voltage of 20 kV, temperature of 25°C and detection wavelength of 280 nm. The relative standard deviations of migration time ranged from 0.2 to 0.7% and the peak area ranged from 1.5 to 3.7% for the seven analytes, indicating the good repeatability of the proposed method. The method was extensively validated by evaluating the linearity (R ≥ 0.9992), limits of detection (0.14-0.36 μg/mL), limits of quantification (0.47-1.19 μg/mL), and recovery (96.0-102.6%). Under the optimum conditions, samples of Danshen and related medicinal plants were analyzed using the developed method with high separation efficiency.
KeywordCapillary zone electrophoresis Danshen Phenolic acids β-cyclodextrin
DOI10.1002/jssc.201400845
URLView the original
Language英語
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Northwestern University Feinberg School of Medicine
2.Universidade de Macau
Recommended Citation
GB/T 7714
Cao J.,We J.,Tian K.,et al. Simultaneous determination of seven phenolic acids in three Salvia species by capillary zone electrophoresis with β-cyclodextrin as modifier[J]. Journal of Separation Science,2014,37(24):3738-3744.
APA Cao J.,We J.,Tian K.,Su H.,Wan J.,&Li P..(2014).Simultaneous determination of seven phenolic acids in three Salvia species by capillary zone electrophoresis with β-cyclodextrin as modifier.Journal of Separation Science,37(24),3738-3744.
MLA Cao J.,et al."Simultaneous determination of seven phenolic acids in three Salvia species by capillary zone electrophoresis with β-cyclodextrin as modifier".Journal of Separation Science 37.24(2014):3738-3744.
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