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Optimization of micellar electrokinetic chromatography method for the simultaneous determination of seven hydrophilic and four lipophilic bioactive components in three salvia species
Cao J.1; Hu J.1; Wei J.1; Li B.2; Zhang M.2; Xiang C.2; Li P.1
2015-08-01
Source PublicationMolecules
ISSN14203049
Volume20Issue:8Pages:15304-15318
AbstractA micellar electrokinetic chromatography (MEKC) method was developed for the simultaneous determination of seven hydrophilic phenolic acids and four lipophilic tanshinones in three Salvia species. In normal MEKC mode using SDS as surfactant, the investigated 11 compounds could not be well separated. Therefore, several buffer modifiers including β-cyclodextrins (β-CD), ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) and organic solvents have been added to the buffer solution to improve the separation selectivity. Under the optimized conditions (BGE, 15 mM sodium tetraborate with 10 mM SDS, 5 mM β-CD, 10 mM [bmim]BF4 and 15% ACN (v/v) as additives; buffer pH, 9.8; voltage, 20 kV; temperature, 25 °C), the 11 investigated analytes could achieve baseline separation in 34 min. The proposed MEKC was additionally validated by evaluating the linearity (R2 ≥ 0.9965), LODs (0.27-1.39 μgmL-1), and recovery (94.26%-105.17%), demonstrating this method was reproducible, accurate and reliable. Moreover, the contents of the 11 compounds in three Salvia species, including S. miltiorrhiza, S. przewalskii and S. castanea were analyzed. The result showed that the established MEKC method was simple and practical for the simultaneous determination of the hydrophilic and lipophilic bioactive components in Salvia species, which could be used to effectively evaluate the quality of these valued medicinal plants.
KeywordBuffer modifiers Micellar electrokinetic chromatography Phenolic acids Salvia species Tanshinones
DOI10.3390/molecules200815304
URLView the original
Language英語
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade de Macau
2.Kunming University of Science and Technology
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Cao J.,Hu J.,Wei J.,et al. Optimization of micellar electrokinetic chromatography method for the simultaneous determination of seven hydrophilic and four lipophilic bioactive components in three salvia species[J]. Molecules,2015,20(8):15304-15318.
APA Cao J..,Hu J..,Wei J..,Li B..,Zhang M..,...&Li P..(2015).Optimization of micellar electrokinetic chromatography method for the simultaneous determination of seven hydrophilic and four lipophilic bioactive components in three salvia species.Molecules,20(8),15304-15318.
MLA Cao J.,et al."Optimization of micellar electrokinetic chromatography method for the simultaneous determination of seven hydrophilic and four lipophilic bioactive components in three salvia species".Molecules 20.8(2015):15304-15318.
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