UM
Separation and Determination of Four Tanshinones in Danshen and Related Medicinal Plants by Micellar Electrokinetic Chromatography Using Ionic Liquids as Modifier
Cao J.1; Wei J.1; Xiang C.2; Zhang M.2; Li B.2; Wan J.1; Su H.1; Li P.1
2016-09-01
Source PublicationJournal of Chromatographic Science
ISSN00219665
Volume54Issue:8Pages:1435-1444
AbstractA simple and fast micellar electrokinetic chromatography (MEKC) method using ionic liquids as modifier was established for simultaneous determination of four hydrophobic tanshinones, including dihydrotanshinone I (1), cryptotanshinone (2), tanshinone I (3) and tanshinone IIA (4), in Danshen and related medicinal plants. In normal MEKC using sodium dodecyl sulfate (SDS) as surfactant and organic solvents as additives, the four tanshinones, especially cryptotanshinone and tanshinone I, could not be well separated. Fortunately, further addition of ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF) resulted in a baseline separation of these four analytes. After an optimization study, 10 mM borax-10 mM SDS-10 mM [bmim]BF containing 15% acetonitrile (v/v) at pH 9.6 was adopted as the running buffer to complete the separation within 16 min at the voltage of 25 kV, temperature of 25°C and detection wavelength of 254 nm. The relative standard deviations of migration time and peak area were in the range of 0.62-2.21 and 1.33-3.90%, respectively, indicating the good repeatability of the developed method. This method was extensively validated by evaluating the linearity (R ≥ 0.9992), limits of detection (0.75-1.11 μg mL), limits of quantification (2.26-3.32 μg mL) and recovery (96.11-103.74%). Under the optimum conditions, samples of Danshen and related medicinal plants were well analyzed with high separation efficiency.
DOI10.1093/chromsci/bmw059
URLView the original
Language英語
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade de Macau
2.Kunming University of Science and Technology
Recommended Citation
GB/T 7714
Cao J.,Wei J.,Xiang C.,et al. Separation and Determination of Four Tanshinones in Danshen and Related Medicinal Plants by Micellar Electrokinetic Chromatography Using Ionic Liquids as Modifier[J]. Journal of Chromatographic Science,2016,54(8):1435-1444.
APA Cao J..,Wei J..,Xiang C..,Zhang M..,Li B..,...&Li P..(2016).Separation and Determination of Four Tanshinones in Danshen and Related Medicinal Plants by Micellar Electrokinetic Chromatography Using Ionic Liquids as Modifier.Journal of Chromatographic Science,54(8),1435-1444.
MLA Cao J.,et al."Separation and Determination of Four Tanshinones in Danshen and Related Medicinal Plants by Micellar Electrokinetic Chromatography Using Ionic Liquids as Modifier".Journal of Chromatographic Science 54.8(2016):1435-1444.
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