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Cell imaging of dopamine receptor using agonist labeling iridium(III) complex
Vellaisamy, Kasipandi1; Li, Guodong2; Ko, Chung-Nga1; Zhong, Hai-Jing2; Fatima, Sarwat3; Kwan, Hiu-Yee3; Wong, Chun-Yuen4; Kwong, Wai-Jing1,6; Tan, Weihong5,6; Leung, Chung-Hang2; Ma, Dik-Lung1
2018-02-07
Source PublicationCHEMICAL SCIENCE
ISSN2041-6520
Volume9Issue:5Pages:1119-1125
Abstract

Dopamine receptor expression is correlated with certain types of cancers, including lung, breast and colon cancers. In this study, we report luminescent iridium(III) complexes (11-14) as intracellular dopamine receptor (D1R/D2R) cell imaging agents. Complexes 11 and 13, which are conjugated with a dopamine receptor agonist, showed superior cell imaging characteristics, high stability and low cytotoxicity (> 100 mu M) in A549 lung cancer cells. siRNA knockdown and dopamine competitive assays indicated that complexes 11 and 13 could selectively bind to dopamine receptors (D1R/D2R) in A549 cells. Fluorescence lifetime microscopy demonstrated that complex 13 has a longer luminescence lifetime at the wavelength of 560-650 nm than DAPI and other chromophores in biological fluids. The long luminescence lifetime of complex 13 not only provides an opportunity for efficient dopamine receptor tracking in biological media, but also enables the temporal separation of the probe signal from the intense background signal by fluorescence lifetime microscopy for efficient analysis. Complex 13 also shows high photostability, which could allow it to be employed for long-term cellular imaging. Furthermore, complex 13 could selectively track the internalization process of dopamine receptors (D1R/D2R) in living cells. To the best of our knowledge, complex 13 is the first metal-based compound that has been used to monitor intracellular dopamine receptors in living cells.

DOI10.1039/c7sc04798c
URLView the original
Indexed BySCI ; IC
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000424010600004
PublisherROYAL SOC CHEMISTRY
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:66   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorKwong, Wai-Jing; Tan, Weihong; Leung, Chung-Hang; Ma, Dik-Lung
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China
3.School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, China
4.Department of Biology and Chemistry, City University of Hong Kong, Kowloon Tong, China
5.Department of Chemistry, Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute, McKnight Brain Institute, University of Florida, Gainesville, USA
6.Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Vellaisamy, Kasipandi,Li, Guodong,Ko, Chung-Nga,et al. Cell imaging of dopamine receptor using agonist labeling iridium(III) complex[J]. CHEMICAL SCIENCE,2018,9(5):1119-1125.
APA Vellaisamy, Kasipandi.,Li, Guodong.,Ko, Chung-Nga.,Zhong, Hai-Jing.,Fatima, Sarwat.,...&Ma, Dik-Lung.(2018).Cell imaging of dopamine receptor using agonist labeling iridium(III) complex.CHEMICAL SCIENCE,9(5),1119-1125.
MLA Vellaisamy, Kasipandi,et al."Cell imaging of dopamine receptor using agonist labeling iridium(III) complex".CHEMICAL SCIENCE 9.5(2018):1119-1125.
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