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Potential target-related proteins in rabbit platelets treated with active monomers dehydrocorydaline and canadine from Rhizoma corydalis
Tan C.-N.1; Zhang Q.1; Li C.-H.1; Fan J.-J.1; Yang F.-Q.1; Hu Y.-J.2; Hu G.3
2019-02-15
Source PublicationPhytomedicine
ISSN1618095X 09447113
Volume54Pages:231-239
Abstract

Background: Dehydrocorydaline (DHC) and canadine (THB) are two active alkaloid compounds in Corydalis yanhusuo (Y.H. Chou & Chun C. Hsu) W.T. Wang ex Z.Y. Su & C.Y. Wu (Papaveraceae) (Rhizoma Corydalis). DHC and THC were previously shown to exert anti-platelet aggregation effect dose-dependently, but their exact mechanisms had not yet been addressed. Therefore, it is essential to study the mechanisms of DHC and THB affecting on platelet's function. Purpose: To investigate the anti-platelet effects and corresponding signal cascades of DHC and THB on platelet aggregation. Methods: Firstly, in vitro anti-platelet aggregation of DHC and THB induced by different agonists including thrombin (THR), adenosine diphosphate (ADP) and arachidonic acid (AA) were determined through turbidimetry method. Then the possible target-related platelet proteins after treated with DHC/THB were separated and identified by two dimensional gel electrophoresis (2-DE) and MALDI-TOF-MS/MS analysis, respectively. Finally, the signal cascades network induced by DHC/THB were predicted through functional analysis of these proteins along with the determination of platelet DAG concentration. Results: The platelet aggregation stimulated by THR, ADP and AA were inhibited by DHC and THB dose-dependently to a certain degree. Meanwhile, DHC and THB had the strongest effect on ADP- and THR-induced platelet aggregation respectively. In addition, treatment of these two compounds caused regulations of about sixty proteins in platelet, including cytoskeleton proteins, cell signaling proteins, proteins related to material energy metabolism, etc. Conclusions: Using proteomic analysis combined with platelet aggregation test and ELISA, this study was successful in exploring the possible mechanisms of DHC/THB on platelet aggregation. DHC might inhibit platelet aggregation by a mechanism involving the ADP receptors P2Y1 and P2Y12, and the effect of THB on platelet function may be related to its binding to THR receptor PAR1 for mediated Gi signaling pathway. These results provide fundamental information for the anti-thrombotic effect of RC.

KeywordCanadine Dehydrocorydaline Proteomic Analysis Rabbit Platelet Signal Cascades Network
DOI10.1016/j.phymed.2018.09.200
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000460119500023
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYang F.-Q.
Affiliation1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China
3.School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China
Recommended Citation
GB/T 7714
Tan C.-N.,Zhang Q.,Li C.-H.,et al. Potential target-related proteins in rabbit platelets treated with active monomers dehydrocorydaline and canadine from Rhizoma corydalis[J]. Phytomedicine,2019,54:231-239.
APA Tan C.-N..,Zhang Q..,Li C.-H..,Fan J.-J..,Yang F.-Q..,...&Hu G..(2019).Potential target-related proteins in rabbit platelets treated with active monomers dehydrocorydaline and canadine from Rhizoma corydalis.Phytomedicine,54,231-239.
MLA Tan C.-N.,et al."Potential target-related proteins in rabbit platelets treated with active monomers dehydrocorydaline and canadine from Rhizoma corydalis".Phytomedicine 54(2019):231-239.
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