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Robust Neuritogenesis-Promoting Activity by Bis(heptyl)-Cognitin Through the Activation of alpha7-Nicotinic Acetylcholine Receptor/ERK Pathway
Hu S.-Q.1; Cui W.1; Mak S.-H.1; Choi C.-L.1; Hu Y.-J.5; Li G.2; Tsim K.W.-K.3; Pang Y.-P.4; Han Y.-F.1
2015-06-01
Source PublicationCNS Neuroscience and Therapeutics
ISSN17555949 17555930
Volume21Issue:6Pages:520-529
AbstractAims: Neurodegenerative disorders are caused by progressive neuronal loss in the brain, and hence, compounds that could promote neuritogenesis may have therapeutic values. In this study, the effects of bis(heptyl)-cognitin (B7C), a multifunctional dimer, on neurite outgrowth were investigated in both PC12 cells and primary cortical neurons. Methods: Immunocytochemical staining was used to evaluate the proneuritogenesis effects, and Western blot and short hairpin RNA assays were applied to explore the underlying mechanisms. Results: B7C (0.1-0.5 μM) induced robust neurite outgrowth in PC12 cells, as evidenced by the neurite-bearing morphology and upregulation of growth-associated protein-43 expression. In addition, B7C markedly promoted neurite outgrowth in primary cortical neurons as shown by the increase in the length of β-III-tubulin-positive neurites. Furthermore, B7C rapidly increased ERK phosphorylation. Specific inhibitors of alpha7-nicotinic acetylcholine receptor (α7-nAChR) and MEK, but not those of p38 or JNK, blocked the neurite outgrowth as well as ERK phosphorylation induced by B7C. Most importantly, genetic depletion of α7-nAChR significantly abolished B7C-induced neurite outgrowth in PC12 cells. Conclusion: B7C promoted neurite outgrowth through the activation of α7-nAChR/ERK pathway, which offers novel insight into the potential application of B7C in the treatment of neurodegenerative disorders.
KeywordAlpha7-nicotinic acetylcholine receptor Bis(heptyl)-cognitin Extracellular signal-regulated kinase Neurite outgrowth Neurodegenerative disorders
DOI10.1111/cns.12401
URLView the original
Language英語
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Hong Kong Polytechnic University
2.Soochow University
3.Hong Kong University of Science and Technology
4.Mayo Clinic
5.Universidade de Macau
6.University of Jinan
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Hu S.-Q.,Cui W.,Mak S.-H.,et al. Robust Neuritogenesis-Promoting Activity by Bis(heptyl)-Cognitin Through the Activation of alpha7-Nicotinic Acetylcholine Receptor/ERK Pathway[J]. CNS Neuroscience and Therapeutics,2015,21(6):520-529.
APA Hu S.-Q..,Cui W..,Mak S.-H..,Choi C.-L..,Hu Y.-J..,...&Han Y.-F..(2015).Robust Neuritogenesis-Promoting Activity by Bis(heptyl)-Cognitin Through the Activation of alpha7-Nicotinic Acetylcholine Receptor/ERK Pathway.CNS Neuroscience and Therapeutics,21(6),520-529.
MLA Hu S.-Q.,et al."Robust Neuritogenesis-Promoting Activity by Bis(heptyl)-Cognitin Through the Activation of alpha7-Nicotinic Acetylcholine Receptor/ERK Pathway".CNS Neuroscience and Therapeutics 21.6(2015):520-529.
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