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Online comprehensive two-dimensional hydrophilic interaction chromatography x reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza
Cao, Ji-Liang; Wang, Song-Song; Hu, Hao; He, Cheng-Wei; Wan, Jian-Bo; Su, Huan-Xing; Wang, Yi-Tao; Li, Peng
2018-02-09
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1536Pages:216-227
Abstract

In the present study, a system was developed for the analysis of phenolic acids in Salvia miltiorrhiza using online comprehensive two-dimensional hydrophilic interaction chromatography and reversed-phase liquid chromatography coupled to a DAD detector and hybrid linear ion trap-Orbitrap mass spectrometry (HILIC x RP-DAD-ESI/HRMS/MSn). The system was configured based on the combination of an XBridge Amide column (150 mm x 4.6 mm, 3.5 mu m) and Accucore PFP column (50 mm x 4.6 mm, 2.6 mu m) for the first and second dimensions, respectively. An additional LC pump was used to dilute the eluent from the first dimension to decrease its elution strength in the second dimension. A back-flush trap column was selected as an interface to make up for the loss of efficiency and resolution due to the online dilution. Under the optimized conditions, a total of 196 peaks of polar compounds were successfully separated and detected in Salvia miltiorrhiza using the developed online HILIC x RP system, which exhibited high orthogonality (73%). The online combination of HILIC and RP provides powerful separation capability for the analysis of polar compounds in samples with complex matrices. (C) 2017 Elsevier B.V. All rights reserved.

KeywordOnline Hilic x Rplc Interfaces Ltq-orbitrap Mass Spectrometry Phenolic Acids Danshen
DOIhttp://doi.org/10.1016/j.chroma.2017.09.041
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000425074200023
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi, Peng
AffiliationState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau 999078, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Cao, Ji-Liang,Wang, Song-Song,Hu, Hao,et al. Online comprehensive two-dimensional hydrophilic interaction chromatography x reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1536:216-227.
APA Cao, Ji-Liang.,Wang, Song-Song.,Hu, Hao.,He, Cheng-Wei.,Wan, Jian-Bo.,...&Li, Peng.(2018).Online comprehensive two-dimensional hydrophilic interaction chromatography x reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza.JOURNAL OF CHROMATOGRAPHY A,1536,216-227.
MLA Cao, Ji-Liang,et al."Online comprehensive two-dimensional hydrophilic interaction chromatography x reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza".JOURNAL OF CHROMATOGRAPHY A 1536(2018):216-227.
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