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Online screening of nitric oxide scavengers in natural products using high performance liquid chromatography coupled with tandem diode array and fluorescence detection
Li D.1; Wang T.1; Guo Y.1; Hu Y.2; Yu B.1; Qi J.1
2015-12-01
Source PublicationJournal of Chromatography A
ISSN18733778 00219673
Volume1425Pages:106-115
AbstractNitric oxide (NO) is an important cellular signaling molecule with extensive physiological and pathophysiological effects. NO scavengers have the potential to treat inflammation, septic shock and other related diseases, and numerous examples have been chemically synthesized or isolated from natural products. The chemical diversity of natural products, however, means that a huge effort is necessary to efficiently screen and identify bioactive compounds, especially NO scavengers. In this article, we propose an effective analytical method to screen for NO scavengers in three natural products using an online system that couples high performance liquid chromatography with tandem diode array and fluorescence detection (HPLC-DAD-FLD). Eighteen compounds from radix of Scutellaria baicalensis Georgi and green tea displayed significant NO scavenging activity whereas components of Pueraria lobata (Willd.) Ohwi had no discernable activity. The structures of the active compounds were elucidated using Agilent Accurate-Mass Q-TOF LC/MS system. Preliminary analysis of structure–activity relationships indicated that, in flavonoids, a 2,3-double bond and a 3-H atom or a 3-OH group are essential for activity. In tannins, poly-hydroxyl groups are important for NO scavenging activity. Method validation indicated that the newly developed method is both reliable and repeatable. The online method that we present provides a simple, rapid and effective way to identify and characterize NO scavengers present in natural products.
KeywordNatural products Nitric oxide Online HPLC-DAD-FLD system Scavenger Screening
DOI10.1016/j.chroma.2015.10.095
URLView the original
Language英語
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.China Pharmaceutical University
2.Universidade de Macau
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GB/T 7714
Li D.,Wang T.,Guo Y.,et al. Online screening of nitric oxide scavengers in natural products using high performance liquid chromatography coupled with tandem diode array and fluorescence detection[J]. Journal of Chromatography A,2015,1425:106-115.
APA Li D.,Wang T.,Guo Y.,Hu Y.,Yu B.,&Qi J..(2015).Online screening of nitric oxide scavengers in natural products using high performance liquid chromatography coupled with tandem diode array and fluorescence detection.Journal of Chromatography A,1425,106-115.
MLA Li D.,et al."Online screening of nitric oxide scavengers in natural products using high performance liquid chromatography coupled with tandem diode array and fluorescence detection".Journal of Chromatography A 1425(2015):106-115.
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