UM
Screening of peroxynitrite scavengers in Flos Lonicerae by using two new methods, an HPLC-DAD-CL technique and a peroxynitrite spiking test followed by HPLC-DAD analysis
Qi J.2; Chen Y.-H.2; Wang Y.2; Chen X.2; Wang L.2; Hu Y.-J.1; Yu B.-Y.2
2016
Source PublicationPhytochemical Analysis
ISSN10991565 09580344
Volume27Issue:1Pages:57-63
AbstractIntroduction Peroxynitrite is involved in the pathogenesis of a number of significant diseases. Peroxynitrite scavengers thus have potential application in understanding and treating these diseases. It is, therefore, important to establish screening methods able to rapidly identify peroxynitrite scavengers from herbal plants. Objective To develop effective and easily operable screening methods for identifying peroxynitrite scavengers in complex matrices, including Chinese herbal medicines. Methods Two simple and efficient screening methods have been developed for the identification of natural peroxynitrite scavengers in Flos Lonicerae Japonicae (FLJ). Method I used HPLC-DAD-(luminol-peroxynitrite)-CL techniques combined with Q-TOF MS/MS analysis, while Method II used pre-column reaction of the sample with peroxynitrite, followed by HPLC separation and Q-TOF MS/MS analysis. Results Five peroxynitrite scavengers (neochlorogenic acid, chlorogenic acid, 3,4-O-dicaffeoyl quinic acid, 3,5-O-dicaffeoyl quinic acid and 4,5-O-dicaffeoyl quinic acid) were identified in FLJ using Method I. Besides the compounds identified using Method I, three additional peroxynitrite scavengers (rutin, isoquercitrin and luteoloside) were identified using Method II. Conclusion The two new methods proved to be complementary and the use of these methods should allow rapid detection of peroxynitrite-scavenging natural products from FLJ and other complex matrices.
KeywordFlos Lonicerae Japonicae HPLC-DAD-CL HPLC-ESI-Q-TOF MS/MS peroxynitrite spiking test peroxynitrite-scavenging activity
DOI10.1002/pca.2599
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade de Macau
2.China Pharmaceutical University
Recommended Citation
GB/T 7714
Qi J.,Chen Y.-H.,Wang Y.,et al. Screening of peroxynitrite scavengers in Flos Lonicerae by using two new methods, an HPLC-DAD-CL technique and a peroxynitrite spiking test followed by HPLC-DAD analysis[J]. Phytochemical Analysis,2016,27(1):57-63.
APA Qi J..,Chen Y.-H..,Wang Y..,Chen X..,Wang L..,...&Yu B.-Y..(2016).Screening of peroxynitrite scavengers in Flos Lonicerae by using two new methods, an HPLC-DAD-CL technique and a peroxynitrite spiking test followed by HPLC-DAD analysis.Phytochemical Analysis,27(1),57-63.
MLA Qi J.,et al."Screening of peroxynitrite scavengers in Flos Lonicerae by using two new methods, an HPLC-DAD-CL technique and a peroxynitrite spiking test followed by HPLC-DAD analysis".Phytochemical Analysis 27.1(2016):57-63.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qi J.]'s Articles
[Chen Y.-H.]'s Articles
[Wang Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qi J.]'s Articles
[Chen Y.-H.]'s Articles
[Wang Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qi J.]'s Articles
[Chen Y.-H.]'s Articles
[Wang Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.