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Integrating qualitative and quantitative characterization of Prunellae Spica by HPLC-QTOF/MS and HPLC-ELSD
YANG J.1; HU Y.-J.2; YU B.-Y.1; QI J.1
2016-05-01
Source PublicationChinese Journal of Natural Medicines
ISSN18755364
Volume14Issue:5Pages:391-400
AbstractThe present study was designed to analyze the major constituents in Prunellae Spica and establish a method for simultaneous determination of two constituents contained in Prunellae Spica. High performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-QTOF-MS/MS) technique was used to identify the constituents in the extractive of Prunellae Spica. High performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) was used to simultaneously quantify two kinds of constituents contained in Prunellae Spica. Principal component analysis (PCA) was applied to compare the similarity and difference among samples from different regions of China. In the present study, 22 compounds were identified and some new fragmental pathways of triterpenic acids were discovered. An accurate and reliable HPLC-ELSD method was developed and validated for the first time to simultaneously quantify multiple constituents, including rosmarinic acid, maslinic acid, corosolic acid, betulin, oleanolic acid, and ursolic acid in the extract of Prunellae Spica. (PCA) revealed some similarities and differences among different samples from different regions of China. In conclusion, our results from this study would be helpful in establishing a scientific and rational quality control method for Prunellae Spica.
KeywordHPLC-ELSD HPLC-QTOF-MS/MS PCA Prunella vulgaris Prunellae Spica
DOI10.3724/SP.J.1009.2016.00391
URLView the original
Language英語
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被引频次[WOS]:3   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题University of Macau
Affiliation1.China Pharmaceutical University
2.Universidade de Macau
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YANG J.,HU Y.-J.,YU B.-Y.,et al. Integrating qualitative and quantitative characterization of Prunellae Spica by HPLC-QTOF/MS and HPLC-ELSD[J]. Chinese Journal of Natural Medicines,2016,14(5):391-400.
APA YANG J.,HU Y.-J.,YU B.-Y.,&QI J..(2016).Integrating qualitative and quantitative characterization of Prunellae Spica by HPLC-QTOF/MS and HPLC-ELSD.Chinese Journal of Natural Medicines,14(5),391-400.
MLA YANG J.,et al."Integrating qualitative and quantitative characterization of Prunellae Spica by HPLC-QTOF/MS and HPLC-ELSD".Chinese Journal of Natural Medicines 14.5(2016):391-400.
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