UM
Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis
Zhang Q.2; Chen C.2; Wang F.-Q.2; Li C.-H.2; Zhang Q.-H.2; Hu Y.-J.1; Xia Z.-N.2; Yang F.-Q.2
2016-12-01
Source PublicationPharmaceutical Biology
ISSN17445116 13880209
Volume54Issue:12Pages:3113-3120
AbstractContext: The rising problem of atherosclerosis and ischemic heart disease emphasizes the need to look for new antithrombotic components with effective modes of action. Corydalis yanhusuo (Y.H. Chou & Chun C. Hsu) W.T. Wang ex Z.Y. Su & C.Y. Wu (Papaveraceae) (Rhizoma Corydalis) has been used in the traditional medicines for the treatment of cardiovascular disease. Objective: The antiplatelet aggregation compounds in Rhizoma Corydalis were screened to validate its traditional medicinal use. Material and methods: Total alkaloid extract (TAE) of Rhizoma Corydalis was obtained by refluxing 100 g Rhizoma Corydalis powder with 600 mL 70% ethanol, and purified by acidification (20% HCl) and alkalization (5 M NaOH) process. Potential antiplatelet aggregation compounds in TAE were screened by a method involving platelet bio-specific extraction and HPLC-DAD/LC–MS analysis. Further in vitro antiplatelet aggregation activity confirmation of TAE and seven main alkaloids were achieved by turbidimetry method within 3 h after blood collection from rabbit carotid artery, and all the test drugs were at the concentration range of 25–350 μg/mL. Finally, HPLC-DAD was employed for the quantitative determination of seven main components in TAE. Results: Five alkaloids, identified as glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline, can be specifically extracted with platelets. The results indicated that all these five alkaloids can inhibit thrombin-induced platelet aggregation in a low dose (IC of glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline were 49.057, 34.914, 33.547, 84.261 and 54.164 μg/mL, respectively) as compared to TAE (IC=175.426 μg/mL) and aspirin (IC=300.340 μg/mL), while the unbound compounds (palmatine and tetrahydropalmatine) had a very weak antiplatelet effect (IC>200 μg/mL). Discussion and conclusion: This study is the first reported work for antiplatelet components screening in Rhizoma Corydalis. Seven compounds were detected and identified by HPLC-DAD/LC–MS, of which five platelet-targeted compounds were discovered.
KeywordHPLC/LC–MS platelet bio-specific extraction Thromboembolic disease traditional Chinese medicines
DOI10.1080/13880209.2016.1211714
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade de Macau
2.Chongqing University
Recommended Citation
GB/T 7714
Zhang Q.,Chen C.,Wang F.-Q.,et al. Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis[J]. Pharmaceutical Biology,2016,54(12):3113-3120.
APA Zhang Q..,Chen C..,Wang F.-Q..,Li C.-H..,Zhang Q.-H..,...&Yang F.-Q..(2016).Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis.Pharmaceutical Biology,54(12),3113-3120.
MLA Zhang Q.,et al."Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis".Pharmaceutical Biology 54.12(2016):3113-3120.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang Q.]'s Articles
[Chen C.]'s Articles
[Wang F.-Q.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang Q.]'s Articles
[Chen C.]'s Articles
[Wang F.-Q.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang Q.]'s Articles
[Chen C.]'s Articles
[Wang F.-Q.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.