| Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis | |
| Zhang Q.2; Chen C.2; Wang F.-Q.2; Li C.-H.2; Zhang Q.-H.2; Hu Y.-J.1; Xia Z.-N.2; Yang F.-Q.2 | |
| 2016-12-01 | |
| Source Publication | Pharmaceutical Biology
 |
| ISSN | 17445116 13880209 |
| Volume | 54Issue:12Pages:3113-3120 |
| Abstract | Context: The rising problem of atherosclerosis and ischemic heart disease emphasizes the need to look for new antithrombotic components with effective modes of action. Corydalis yanhusuo (Y.H. Chou & Chun C. Hsu) W.T. Wang ex Z.Y. Su & C.Y. Wu (Papaveraceae) (Rhizoma Corydalis) has been used in the traditional medicines for the treatment of cardiovascular disease. Objective: The antiplatelet aggregation compounds in Rhizoma Corydalis were screened to validate its traditional medicinal use. Material and methods: Total alkaloid extract (TAE) of Rhizoma Corydalis was obtained by refluxing 100 g Rhizoma Corydalis powder with 600 mL 70% ethanol, and purified by acidification (20% HCl) and alkalization (5 M NaOH) process. Potential antiplatelet aggregation compounds in TAE were screened by a method involving platelet bio-specific extraction and HPLC-DAD/LC–MS analysis. Further in vitro antiplatelet aggregation activity confirmation of TAE and seven main alkaloids were achieved by turbidimetry method within 3 h after blood collection from rabbit carotid artery, and all the test drugs were at the concentration range of 25–350 μg/mL. Finally, HPLC-DAD was employed for the quantitative determination of seven main components in TAE. Results: Five alkaloids, identified as glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline, can be specifically extracted with platelets. The results indicated that all these five alkaloids can inhibit thrombin-induced platelet aggregation in a low dose (IC of glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline were 49.057, 34.914, 33.547, 84.261 and 54.164 μg/mL, respectively) as compared to TAE (IC=175.426 μg/mL) and aspirin (IC=300.340 μg/mL), while the unbound compounds (palmatine and tetrahydropalmatine) had a very weak antiplatelet effect (IC>200 μg/mL). Discussion and conclusion: This study is the first reported work for antiplatelet components screening in Rhizoma Corydalis. Seven compounds were detected and identified by HPLC-DAD/LC–MS, of which five platelet-targeted compounds were discovered. |
| Keyword | HPLC/LC–MS platelet bio-specific extraction Thromboembolic disease traditional Chinese medicines |
| DOI | 10.1080/13880209.2016.1211714 |
| URL | View the original |
| Language | 英語 |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | 1.Universidade de Macau 2.Chongqing University |
| Recommended Citation GB/T 7714 | Zhang Q.,Chen C.,Wang F.-Q.,et al. Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis[J]. Pharmaceutical Biology,2016,54(12):3113-3120. |
| APA | Zhang Q..,Chen C..,Wang F.-Q..,Li C.-H..,Zhang Q.-H..,...&Yang F.-Q..(2016).Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis.Pharmaceutical Biology,54(12),3113-3120. |
| MLA | Zhang Q.,et al."Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis".Pharmaceutical Biology 54.12(2016):3113-3120. |
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