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Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse
Fu, Xufeng1,2,3; Jiang, Bin4; Zheng, Bingrong2; Yan, Yaping1; Wang, Junfeng5; Duan, Yanchao1; Li, Shanshan1; Yan, Li4; Wang, Hong1; Chen, Bingbing1; Sang, Xiongbo2; Ji, Weizhi1,6; Xu, Ren-He4; Si, Wei1,6
2018-02-12
Source PublicationPEERJ
ISSN2167-8359
Volume6
Abstract

Liver fibrosis is a disease that causes high morbidity and has become a major health problem. Liver fibrosis can lead to the end stage of liver diseases (livercirrhosisand hepatocellularcarcinoma). Currently, liver transplantation is the only effective treatment for end-stage liver disease. However, the shortage of organ donors, high cost of medical surgery, immunological rejection and transplantation complications severely hamper liver transplantation therapy. Mesenchymal stem cells (MSCs) have been regarded as promising cells for clinical applications in stem cell therapy in the treatment of liver diseases due to their unique multipotent differentiation capacity, immunoregulation and paracrine effects. Although liver fibrosis improvements by MSC transplantation in preclinical experiments as well as clinical trials have been reported, the in vivo fate of MSCs after transportation and their therapeutic mechanisms remain unclear. In this present study, we isolated MSCs from the bone marrow of rhesus macaques. The cells exhibited typical MSC markers and could differentiate into chondrocytes, osteocytes, and adipocytes, which were not affected by labeling with enhanced green fluorescent protein (EGFP). The harvested MSCs respond to interferon-gamma stimulation and have the ability to inhibit lymphocyte proliferation in vitro. EGFP-labeled MSCs (1 x 10(6) cells) were transplanted into mice with carbon tetrachloride-induced liver fibrosis via tail vein injection. The ability of the heterogenic MSC infusion to ameliorate liver fibrosis in mice was evaluated by a blood plasma chemistry index, pathological examination and liver fibrosis-associated gene expression. Additionally, a small number of MSCs that homed and engrafted in the mouse liver tissues were evaluated by immunofluorescence analysis. Our results showed that the transplantation of heterogenic MSCs derived from monkey bone marrow can be used to treat liver fibrosis in the mouse model and that the paracrine effects of MSCs may play an important role in the improvement of liver fibrosis.

KeywordMesenchymal Stem Cells Liver Fibrosis Paracrine Effects Rhesus Macaque
DOI10.7717/peerj.4336
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000425029400004
PublisherPEERJ INC
The Source to ArticleWOS
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Co-First AuthorFu, Xufeng; Jiang, Bin
Corresponding AuthorXu, Ren-He; Si, Wei
Affiliation1.Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China
2.School of Medicine, Yunnan University, Kunming, Yunnan, China
3.Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China
4.Faculty of Health Sciences, University of Macau, Taipa, Macau
5.Department of Hepatic and Bile Duct Surgery, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China
6.Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, China
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Fu, Xufeng,Jiang, Bin,Zheng, Bingrong,et al. Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse[J]. PEERJ,2018,6.
APA Fu, Xufeng.,Jiang, Bin.,Zheng, Bingrong.,Yan, Yaping.,Wang, Junfeng.,...&Si, Wei.(2018).Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse.PEERJ,6.
MLA Fu, Xufeng,et al."Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse".PEERJ 6(2018).
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