UM
Metabolic fate analysis of Huang-Lian-Jie-Du Decoction in rat urine and feces by LC-IT-MS combining with LC-FT-ICR-MS: A feasible strategy for the metabolism study of Chinese medical formula
Zuo R.1; Ren W.1; Bian B.-L.1; Wang H.-J.1; Wang Y.-N.3; Hu H.4; Zhao H.-Y.1; Si N.1
2016-01-02
Source PublicationXenobiotica
ISSN13665928 00498254
Volume46Issue:1Pages:65-81
Abstract

1. Huang-Lian-Jie-Du Decoction (HLJDD) is widely used for the treatment of hypertension, diabetes, inflammation and neural system diseases in clinic. In the present study, the comprehensive metabolic profile of HLJDD was demonstrated reliably and rapidly followed by the metabolic pathway analysis of six typical pure compounds (four alkaloids, one flavonoid and one iridoid) in HLJDD using LC-IT-MS combined with high resolution LC-FT-ICR-MS.2. Totally, 85 compounds, including 32 prototype components and 53 biotransformed metabolites were detected and characterized in the urine and feces after oral administration of HLJDD and six pure compounds to rats, respectively. Among them, 17 prototypes were identified definitely with standard references.3. Hydroxylation, demethylation and glucuronidation reactions of alkaloids, as well as glucuronidation and sulfonation reactions of iridoids and flavonoids, were observed as the major metabolic pathways of HLJDD. Flavonoids, iridoids and their metabolites were mainly excreted from urine. However, amount of alkaloids were detected in feces.4. In general, the distinctive metabolic process of three kinds of representative components in HLJDD was clarified. The in vivo metabolic network of HLJDD was demonstrated. Meanwhile, the investigation of representative pure compounds in metabolic study provided a valuable strategy to elucidate the full-scale metabolic fate of HLJDD. This might be helpful to understand the in vivo mechanism of Traditional Chinese medicine (TCM).

KeywordHuang-lian-jie-du Decoction Lc-it-ms Combined With Lc-ft-icr-ms Metabolic Fate Urine And Feces
DOI10.3109/00498254.2015.1048541
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000368026300007
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Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.China Academy of Chinese Medical Sciences
2.Li Kang Hospital
3.Capital Medical University China
4.Universidade de Macau
Recommended Citation
GB/T 7714
Zuo R.,Ren W.,Bian B.-L.,et al. Metabolic fate analysis of Huang-Lian-Jie-Du Decoction in rat urine and feces by LC-IT-MS combining with LC-FT-ICR-MS: A feasible strategy for the metabolism study of Chinese medical formula[J]. Xenobiotica,2016,46(1):65-81.
APA Zuo R..,Ren W..,Bian B.-L..,Wang H.-J..,Wang Y.-N..,...&Si N..(2016).Metabolic fate analysis of Huang-Lian-Jie-Du Decoction in rat urine and feces by LC-IT-MS combining with LC-FT-ICR-MS: A feasible strategy for the metabolism study of Chinese medical formula.Xenobiotica,46(1),65-81.
MLA Zuo R.,et al."Metabolic fate analysis of Huang-Lian-Jie-Du Decoction in rat urine and feces by LC-IT-MS combining with LC-FT-ICR-MS: A feasible strategy for the metabolism study of Chinese medical formula".Xenobiotica 46.1(2016):65-81.
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