UM
Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells
Li J.-H.1; He C.-W.1; Liang N.-C.1; Mo L.-E.2; Zhang X.1
1999-07-10
Source PublicationActa Pharmacologica Sinica
ISSN02539756
Volume20Issue:6Pages:541-545
AbstractAIM: To study the effect of the antitumor compounds 5F, 6F, and A from Pteris semipinnata L on the activities of DNA topoisomerases and cell cycle of HL-60 cells, and the synergism of compound 6F in combination with genistein in vitro. METHODS: DNA topoisomerases were isolated from HL-60 cell lines, and supercoiled pBR322 DNA was used as substrate to determine the activities of DNA topoisomerase I and II. Cell cycle was analyzed by flow cytometry (FCM). Cytotoxicity assay was tested by MTT method. RESULTS: Compounds 5F, 6F, and A inhibited the activities of DNA topoisomerase I and II. After exposure of the cells to compound 6F, an increase in cells in the S and G/M phases and a decrease in cells in the G/G phase of the cell cycle were observed. At low concentrations (57.8 and 115.6 nmol · L), compound 6F enhanced the cytotoxicity against HL-60 cell line in combination with genistein, q values were > 1.15. The enhancement times of 57.8 and 115.6 nmol · L of 6F by genistein were 2.60 and 4.65, respectively. CONCLUSION: Compounds 5F, 6F, and A inhibited the activities of DNA topoisomerases of HL-60 cells. Compound 6F increased the number of cells in S and G/M phases, decreased the population of G/G phase cells, and enhanced the cytotoxicity of genistein, which had synergism with 6F in antitumor action.
KeywordAntineoplastic agents Cell cycle Diterpenes DNA topoisomerase Drag synergism Genistein HL-60 cells Pteris semipinnata L
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Institute of Medical Biochemistry
2.Guangdong Medical University
Recommended Citation
GB/T 7714
Li J.-H.,He C.-W.,Liang N.-C.,et al. Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells[J]. Acta Pharmacologica Sinica,1999,20(6):541-545.
APA Li J.-H.,He C.-W.,Liang N.-C.,Mo L.-E.,&Zhang X..(1999).Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells.Acta Pharmacologica Sinica,20(6),541-545.
MLA Li J.-H.,et al."Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells".Acta Pharmacologica Sinica 20.6(1999):541-545.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li J.-H.]'s Articles
[He C.-W.]'s Articles
[Liang N.-C.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li J.-H.]'s Articles
[He C.-W.]'s Articles
[Liang N.-C.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li J.-H.]'s Articles
[He C.-W.]'s Articles
[Liang N.-C.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.