UM
Study on the diterpenoid constituents and anticancer action of Pteris semipinnata
Zhang X.; Li J.; He C.
1999-08-01
Source PublicationChinese Pharmaceutical Journal
ISSN10012494
Volume34Issue:8Pages:512-514
AbstractOBJECTIVE: To investigate the diterpenoid constituents and anticancer action of the pteris semipinnata L. (PSL). METHOD: The ethanol extract was fractionated over an active charcoal column, eluted with ethanol-chloroform (10:1). After concentrating, the remainder was chromatographed on sillica gel column, eluted with chloroform-methanol (90:2). The pure compounds were obtained. Their structures were confirmed by spectra, espectially by two dimensional nuclear magnetic resonance (2D-NMR) for their stereo structures. Cell viability was determined using the 3-(4,5-Dimethyl-2-thia-zolyl)2,5-diphenyl-2H-tetrazolium bromide (MTT) test, and the IC value were calculated by the modified Karber formula. RESULTS: Five compounds, B, 4F, 5F, 6F and A, were obtained. A is a new compuound, 6F was isolated from the plant for the first time. A and 6F exhibited obvious activity to inhibit the growth of human promyelocytic leukemia cells (HL-60), gastric adenocarcinom cell line (MGC-803), human nasopharyngeal carcinoma cells in low differentiation (CNE-2Z) and human liver adenocarcinoma cell line (BEL-7402), 6F is more active than the positive control by 5Fu, their IC for LC-60 were 0.09 μmol.L and 52.5 μmol.L, respectively. CONCLUSION: The diterpenoids obtained from the PSL possess obvious antitumour activity. The preliminary study on the structural-activity relationship suggested that the cytotoxic activity is related to the α-methylene cyclopentanone moity. The 6F is the most active one.
KeywordAntitumor Diterpenoid Ent-kaurane Pteris semipinnata L.
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
AffiliationGuangdong Medical University
Recommended Citation
GB/T 7714
Zhang X.,Li J.,He C.. Study on the diterpenoid constituents and anticancer action of Pteris semipinnata[J]. Chinese Pharmaceutical Journal,1999,34(8):512-514.
APA Zhang X.,Li J.,&He C..(1999).Study on the diterpenoid constituents and anticancer action of Pteris semipinnata.Chinese Pharmaceutical Journal,34(8),512-514.
MLA Zhang X.,et al."Study on the diterpenoid constituents and anticancer action of Pteris semipinnata".Chinese Pharmaceutical Journal 34.8(1999):512-514.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang X.]'s Articles
[Li J.]'s Articles
[He C.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang X.]'s Articles
[Li J.]'s Articles
[He C.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang X.]'s Articles
[Li J.]'s Articles
[He C.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.