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Inhibitory effect of compound 6F isolated from Pteris semipinnata L. on the activity of protein kinase C in HL-60 cells
He C.-W.; Liang N.-C.; Mo L.-E.; Li J.-H.; Zhang X.
2003-04-01
Source PublicationChinese Journal of Pharmacology and Toxicology
ISSN10003002
Volume17Issue:2Pages:81-86
AbstractAIM: To investigate whether compound 6F isolated from Pteris semipinnata L inhibits the activity of protein kinase C (PKC) and whether the DNA fragment induction and cytotoxicity of 6F on HL-60 cells relate to PKC signaling pathway. METHODS: HL-60 cells were used as in vitro model and its cytosol (soluble sample) and particle(insoluble sample including membrane system and nuclei) fractions obtained by ultracentrifugation were used as samples for PKC assay. PKC activity was measured by incorporation of [γ-P]ATP into exogenous substrate after stimulated by phosphatidylserine and diolein. Diphenylamine assay and MTT staining methods were applied for DNA fragmentation detection and cytotoxicity assay, respectively. RESULTS: PKC activities in cytosol and particle fractions were inhibited by 6F in a dose-dependent manner(P < 0.05 in the series of cytosol fraction, P < 0.01 in the series of particle fraction). Phorbol 12-myristate 13-acetate(PMA, 65 nmol·L) partially attenuated the DNA fragmentation induced by 6F in HL-60 cells, and protected cells against the cytotoxic effect of 6F (P < 0.01). The ability of reducing MTT to formazan in mitochondria of HL-60 cells was potentiated when the cells were treated with PMA alone at the same concentration for 24 h (P < 0.01). CONCLUSION: Compound 6F is an inhibitor for PKC. DNA fragment induction and cytotoxic effect of 6F may be partially through its inhibitory effect on PKC activity.
KeywordCytotoxicity DNA fragmentation Protein kinase C Pteris semipinnata L.
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Language英語
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Document TypeJournal article
专题Institute of Chinese Medical Sciences
AffiliationGuangdong Medical University
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GB/T 7714
He C.-W.,Liang N.-C.,Mo L.-E.,et al. Inhibitory effect of compound 6F isolated from Pteris semipinnata L. on the activity of protein kinase C in HL-60 cells[J]. Chinese Journal of Pharmacology and Toxicology,2003,17(2):81-86.
APA He C.-W.,Liang N.-C.,Mo L.-E.,Li J.-H.,&Zhang X..(2003).Inhibitory effect of compound 6F isolated from Pteris semipinnata L. on the activity of protein kinase C in HL-60 cells.Chinese Journal of Pharmacology and Toxicology,17(2),81-86.
MLA He C.-W.,et al."Inhibitory effect of compound 6F isolated from Pteris semipinnata L. on the activity of protein kinase C in HL-60 cells".Chinese Journal of Pharmacology and Toxicology 17.2(2003):81-86.
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