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Label-free biomedical imaging with high sensitivity by stimulated raman scattering microscopy
Freudiger C.W.1; Min W.1; Saar B.G.1; Lu S.1; Holtom G.R.1; He C.2; Tsai J.C.3; Kang J.X.2; Xie X.S.1
2008-12-19
Source PublicationScience
ISSN00368075 10959203
Volume322Issue:5909Pages:1857-1861
Abstract

Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and readily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast, and monitoring drug delivery through the epidermis.

DOI10.1126/science.1165758
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000261799400059
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Cited Times [WOS]:1065   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorXie X.S.
Affiliation1.Harvard University
2.Massachusetts General Hospital
3.Pfizer Inc.
Recommended Citation
GB/T 7714
Freudiger C.W.,Min W.,Saar B.G.,et al. Label-free biomedical imaging with high sensitivity by stimulated raman scattering microscopy[J]. Science,2008,322(5909):1857-1861.
APA Freudiger C.W..,Min W..,Saar B.G..,Lu S..,Holtom G.R..,...&Xie X.S..(2008).Label-free biomedical imaging with high sensitivity by stimulated raman scattering microscopy.Science,322(5909),1857-1861.
MLA Freudiger C.W.,et al."Label-free biomedical imaging with high sensitivity by stimulated raman scattering microscopy".Science 322.5909(2008):1857-1861.
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