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Protection against oxygen-glucose deprivation/reperfusion injury in cortical neurons by combining omega-3 polyunsaturated acid with Lyciumbarbarum polysaccharide
Shi Z.2; Wu D.2; Yao J.-P.1; Yao X.1; Huang Z.2; Li P.2; Wan J.-B.2; He C.2; Su H.2
2016-01-13
Source PublicationNutrients
ISSN20726643
Volume8Issue:1
Abstract

Ischemic stroke, characterized by the disturbance of the blood supply to the brain, is a severe worldwide health threat with high mortality and morbidity. However, there is no effective pharmacotherapy for ischemic injury. Currently, combined treatment is highly recommended for this devastating injury. In the present study, we investigated neuroprotective effects of the combination of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) and Lyciumbarbarum polysaccharide (LBP) on cortical neurons using an in vitro ischemic model. Our study demonstrated that treatment with docosahexaenoic acid (DHA), a major component of the ω-3 PUFAs family, significantly inhibited the increase of intracellular Ca in cultured wild type (WT) cortical neurons subjected to oxygen-glucose deprivation/reperfusion (OGD/R) injury and promoted their survival compared with the vehicle-treated control. The protective effects were further confirmed in cultured neurons with high endogenous ω-3 PUFAs that were isolated from fat-1 mice, in that a higher survival rate was found in fat-1 neurons compared with wild-type neurons after OGD/R injury. Our study also found that treatment with LBP (50 mg/L) activated Trk-B signaling in cortical neurons and significantly attenuated OGD/R-induced cell apoptosis compared with the control. Notably, both combining LBP treatment with ω-3 PUFAs administration to WT neurons and adding LBP to fat-1 neurons showed enhanced effects on protecting cortical neurons against OGD/R injury via concurrently regulating the intracellular calcium overload and neurotrophic pathway. The results of the study suggest that ω-3 PUFAs and LBP are promising candidates for combined pharmacotherapy for ischemic stroke.

KeywordCa2++ Cortical Neurons Dha Lbp Neuroprotection Ogd/r Trk-b
DOI10.3390/nu8010041
URLView the original
WOS Research AreaNutrition & Dietetics
WOS SubjectNutrition & Dietetics
WOS IDWOS:000374589300040
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被引频次[WOS]:6   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Corresponding AuthorSu H.
Affiliation1.Sun Yat-Sen University
2.Universidade de Macau
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Shi Z.,Wu D.,Yao J.-P.,et al. Protection against oxygen-glucose deprivation/reperfusion injury in cortical neurons by combining omega-3 polyunsaturated acid with Lyciumbarbarum polysaccharide[J]. Nutrients,2016,8(1).
APA Shi Z..,Wu D..,Yao J.-P..,Yao X..,Huang Z..,...&Su H..(2016).Protection against oxygen-glucose deprivation/reperfusion injury in cortical neurons by combining omega-3 polyunsaturated acid with Lyciumbarbarum polysaccharide.Nutrients,8(1).
MLA Shi Z.,et al."Protection against oxygen-glucose deprivation/reperfusion injury in cortical neurons by combining omega-3 polyunsaturated acid with Lyciumbarbarum polysaccharide".Nutrients 8.1(2016).
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