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Protective effects of Otophylloside N on Pentylenetetrazol-induced neuronal injury in vitro and in vivo
Sheng F.1; Chen M.1; Tan Y.1; Xiang C.2; Zhang M.2; Li B.2; Su H.1; He C.1; Wan J.1; Li P.1
2016-07-25
Source PublicationFrontiers in Pharmacology
ISSN16639812
Volume7Issue:JUL
Abstract

Approximately 30% of epileptic patients worldwide are medically unable to control their seizures. In addition, repeated epileptic seizures generally lead to neural damage. Pentylenetetrazol (PTZ) is a clinical circulatory and respiratory stimulant that is experimentally used to mimic epileptic convulsion in epilepsy research. Here, we systematically explore the neuroprotective effects of a pure compound isolated from Cynanchum otophyllum Schneid (Qingyangshen), Otophylloside N (OtoN), against PTZ-induced neuronal injury. We used three models: in vitro primary cortical neurons, in vivo mice, and in vivo zebrafish. Our results revealed that OtoN treatment may attenuate PTZ-induced morphology changes, cell death, LDH efflux in embryonic neuronal cells of C57BL/6J mice, and convulsive behavior in zebrafish. Additionally, our Western blot and RT-PCR results demonstrated that OtoN may attenuate PTZ-induced apoptosis and neuronal activation in neuronal cells, mice, and zebrafish. OtoN may reduce PTZ-induced cleavage of poly ADP-ribose polymerase and upregulation of the Bax/Bcl-2 ratio and decrease the expression level of c-Fos. This study is the first investigation of the neuroprotective effects of OtoN, which might be developed as a novel antiepileptic drug.

KeywordCynanchum Otophyllum Schneid Epilepsy Neuroprotective Effect, Apoptosis Otophylloside n Pentylenetetrazol
DOI10.3389/fphar.2016.00224
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000380094600002
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi B.; Li P.
Affiliation1.Universidade de Macau
2.Kunming University of Science and Technology
Recommended Citation
GB/T 7714
Sheng F.,Chen M.,Tan Y.,et al. Protective effects of Otophylloside N on Pentylenetetrazol-induced neuronal injury in vitro and in vivo[J]. Frontiers in Pharmacology,2016,7(JUL).
APA Sheng F..,Chen M..,Tan Y..,Xiang C..,Zhang M..,...&Li P..(2016).Protective effects of Otophylloside N on Pentylenetetrazol-induced neuronal injury in vitro and in vivo.Frontiers in Pharmacology,7(JUL).
MLA Sheng F.,et al."Protective effects of Otophylloside N on Pentylenetetrazol-induced neuronal injury in vitro and in vivo".Frontiers in Pharmacology 7.JUL(2016).
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