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A sensitive method for determination of platycodin D in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study
Pei L.3; Bao Y.3; Ma L.1; Wang Q.1; Ye Y.3; Han X.3; Liu S.3; Chen X.2
2012
Source PublicationPlanta Medica
ISSN00320943 14390221
Volume78Issue:3Pages:244-251
Abstract

Platycodin D (PD), a major component isolated from the root of Platycodon grandiflorum, is widely used in traditional Chinese medicine. A sensitive rapid analytical method was established and validated to determine the PD in rat plasma. This method was further applied to assess the pharmacokinetics of PD in rats following administration of a single dose. Liquid chromatography tandem mass spectrometry (LC/MS/MS) in multiple reaction monitoring mode (MRM) was used in the method, and tubeimoside I was used as the internal standard (IS). A simple protein precipitation based on methanol (MeOH) was employed. The combination of a simple sample cleanup and short chromatographic running time (4min) increased the throughput of the method substantially. The method was validated over the range of 0.51000ng/mL with a correlation coefficient >0.99. The lower limit of quantification was 0.5ng/mL for PD in plasma. Intra- and inter-day accuracies for PD were 90115% and 96108%, respectively, and the inter-day precision was less than 15%. After a single oral dose of 10mg/kg of PD, its mean peak plasma concentration (C) was 13.7±4.5ng/mL at 0.5h. The area under the plasma concentration-time curve (AUC) was 35.4±16.1hng/mL, and the elimination half-life (T) was 1.48±0.13h. In case of intravenous administration of PD at a dosage of 0.5mg/kg, the area under the plasma concentration-time curve (AUC) was 2203±258hng/mL, and the elimination half-life (T) was 6.57±0.70h. Based on the results, the oral bioavailability of PD in rats at 10mg/kg is 0.079%. © Georg Thieme Verlag KG Stuttgart - New York.

KeywordCampanulaceae Lc/ms/ms Pharmacokinetics Platycodin d PlA.ycodon GrA.diflorum A. Dc Tubeimoside i
DOI10.1055/s-0031-1280372
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000300640100008
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Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.East China University of Science and Technology
2.Universidade de Macau
3.Shanghai University of Traditional Chinese Medicine
Recommended Citation
GB/T 7714
Pei L.,Bao Y.,Ma L.,et al. A sensitive method for determination of platycodin D in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study[J]. Planta Medica,2012,78(3):244-251.
APA Pei L..,Bao Y..,Ma L..,Wang Q..,Ye Y..,...&Chen X..(2012).A sensitive method for determination of platycodin D in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study.Planta Medica,78(3),244-251.
MLA Pei L.,et al."A sensitive method for determination of platycodin D in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study".Planta Medica 78.3(2012):244-251.
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