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A sensitive method for determination of furanodiene in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study
Pei L.2; Liu S.2; Zheng J.2; Chen X.1
2012-07-01
Source PublicationBiomedical Chromatography
ISSN02693879 10990801
Volume26Issue:7Pages:826-832
Abstract

Furanodiene, a sesquiterpene component extracted from the essential oil of the rhizome of Curcuma wenyujin Y.H. Chen et C. Ling (Wen Ezhu), is widely used in traditional Chinese medicine. A sensitive analytical method was established and validated for furanodiene in rat plasma, which was further applied to assess the pharmacokinetics of furanodiene in rats receiving a single dose of furanodiene. Liquid chromatography tandem mass spectrometry (LC/MS/MS) in multiple reaction monitoring mode was used in the method and costundide was used as internal standard. A simple protein precipitation based on methanol was employed. The simple sample cleanup increased the throughput of the method substantially. The method was validated over the range of 1-1000ng/mL with a correlation coefficient >0.99. The lower limit of quantification was 1ng/mL for furanodiene in plasma. Intra- and inter-day accuracies for furanodiene were 88-115 and 102-107%, and the inter-day precision less than 14.4%. After a single oral dose of 10mg/kg of furanodiene, the mean peak plasma concentration of furanodiene was 66.9±23.4ng/mL at 1h, the area under the plasma concentration-time curve (AUC ) was 220±47.8h ng/mL, and the elimination half-life was 1.53±0.06h. After an intravenous adminstration of furanodiene at a dosage of 5mg/kg, the area under the plasma concentration-time curve was 225±76.1h{dot operator}ng/mL, and the elimination half-life was 2.40±1.18h. Based on this result, the oral bioavailability of furanodiene in rats at 10mg/kg is 49.0%. © 2011 John Wiley & Sons, Ltd.

KeywordCostundide Furanodiene Lc/ms/ms Pharmacokinetics
DOI10.1002/bmc.1736
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000304989300007
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Universidade de Macau
2.Shanghai University of Traditional Chinese Medicine
Recommended Citation
GB/T 7714
Pei L.,Liu S.,Zheng J.,et al. A sensitive method for determination of furanodiene in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study[J]. Biomedical Chromatography,2012,26(7):826-832.
APA Pei L.,Liu S.,Zheng J.,&Chen X..(2012).A sensitive method for determination of furanodiene in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study.Biomedical Chromatography,26(7),826-832.
MLA Pei L.,et al."A sensitive method for determination of furanodiene in rat plasma using liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study".Biomedical Chromatography 26.7(2012):826-832.
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