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Cucurbitacin B inhibits proliferation, induces G2/M cycle arrest and autophagy without affecting apoptosis but enhances MTT reduction in PC12 cells
Wu C.; Bao J.; He C.; Lu J.; Chen X.
2016
Source PublicationBangladesh Journal of Pharmacology
ISSN19910088 1991007X
Volume11Issue:1Pages:110-118
Abstract

In the present study, the effect of cucurbitacin B (a natural product with anticancer effect) was studied on PC12 cells. It significantly reduced the cell number, changed cell morphology and inhibited colony formation while MTT results showed increased cell viability. Cucurbitacin B treatment increased activity of succinode hydrogenase. No alteration in the integrity of membrane, the release of lactic dehydrogenase, the mitochondrial membrane potential, and the expression of apoptotic proteins suggested that cucurbitacin B did not induce apoptosis. The cell cycle was remarkably arrested at G2/M phase. Furthermore, cucurbitacin B induced autophagy as evidence by accumulation of autophagic vacuoles and the increase of LC3II. In addition, cucurbitacin B up-regulated the expression of p-beclin-1, p-ULK1, p-Wee1, p21 and down-regulated p-mTOR, p-p70S6K, CDC25C, CDK1, Cyclin B1. In conclusion, cucurbitacin B inhibited PC12 proliferation but caused MTT pitfall. Cucurbitacin B induced G2/M cell cycle arrest, autophagy, but not the apoptosis in PC12 cells.

KeywordApoptosis Autophagy Cucurbitacin b G2/m Cycle Mtt Pc12 Cell
DOIhttps://www.banglajol.info/index.php/BJP/article/view/23791/0
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000374889400014
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversidade de Macau
Recommended Citation
GB/T 7714
Wu C.,Bao J.,He C.,et al. Cucurbitacin B inhibits proliferation, induces G2/M cycle arrest and autophagy without affecting apoptosis but enhances MTT reduction in PC12 cells[J]. Bangladesh Journal of Pharmacology,2016,11(1):110-118.
APA Wu C.,Bao J.,He C.,Lu J.,&Chen X..(2016).Cucurbitacin B inhibits proliferation, induces G2/M cycle arrest and autophagy without affecting apoptosis but enhances MTT reduction in PC12 cells.Bangladesh Journal of Pharmacology,11(1),110-118.
MLA Wu C.,et al."Cucurbitacin B inhibits proliferation, induces G2/M cycle arrest and autophagy without affecting apoptosis but enhances MTT reduction in PC12 cells".Bangladesh Journal of Pharmacology 11.1(2016):110-118.
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