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Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes
Ding Q.3; Bao J.3; Zhao W.3; Lu J.3; Zhu H.2; Chen X.3
2016-03-01
Source PublicationMolecular and Cellular Toxicology
ISSN20928467 1738642X
Volume12Issue:1Pages:29-36
Abstract

Ethanol is a common risk factor for liver injury. Cucurbitacin B (CuB) is a natural product with potent cytotoxic activities mediated by inducing apoptosis. This study investigated the effect of ethanol on CuB-induced cytotoxicity in LO2 hepatocytes. Low concentration of ethanol alone showed no significant cytotoxic effect on LO2 cells. CuB dose-dependently decreased cell viability. However, ethanol co-treatment significantly enhanced CuB-induced cytotoxicity. CuB-induced mitochondria membrane potential (ΔΨ) depolarization was further decreased by ethanol. Furthermore, CuB-induced apoptosis was augmented by ethanol, as evidenced by DNA fragmentation, Annexin V staining, and apoptotic protein expression. Ethanol inhibited CuB-induced autophagy, as determined by MDC staining, autophagic protein expression, and transmission electron microscopy. Therefore, the present data suggested that ethanol enhanced CuB cytotoxicity in LO2 hepatocytes, which was mediated by inhibiting autophagy and augmenting apoptosis.

KeywordApoptosis Autophagy Cucurbitacin b Ethanol Hepatocyte
DOI10.1007/s13273-016-0005-2
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Toxicology
WOS SubjectBiochemistry & Molecular Biology ; Toxicology
WOS IDWOS:000373095200005
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Guiyang College of Traditional Chinese Medicine
2.Zhejiang University
3.Universidade de Macau
Recommended Citation
GB/T 7714
Ding Q.,Bao J.,Zhao W.,et al. Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes[J]. Molecular and Cellular Toxicology,2016,12(1):29-36.
APA Ding Q.,Bao J.,Zhao W.,Lu J.,Zhu H.,&Chen X..(2016).Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes.Molecular and Cellular Toxicology,12(1),29-36.
MLA Ding Q.,et al."Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes".Molecular and Cellular Toxicology 12.1(2016):29-36.
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