Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes | |
Ding Q.3; Bao J.3; Zhao W.3; Lu J.3![]() ![]() | |
2016-03-01 | |
Source Publication | Molecular and Cellular Toxicology
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ISSN | 20928467 1738642X |
Volume | 12Issue:1Pages:29-36 |
Abstract | Ethanol is a common risk factor for liver injury. Cucurbitacin B (CuB) is a natural product with potent cytotoxic activities mediated by inducing apoptosis. This study investigated the effect of ethanol on CuB-induced cytotoxicity in LO2 hepatocytes. Low concentration of ethanol alone showed no significant cytotoxic effect on LO2 cells. CuB dose-dependently decreased cell viability. However, ethanol co-treatment significantly enhanced CuB-induced cytotoxicity. CuB-induced mitochondria membrane potential (ΔΨ) depolarization was further decreased by ethanol. Furthermore, CuB-induced apoptosis was augmented by ethanol, as evidenced by DNA fragmentation, Annexin V staining, and apoptotic protein expression. Ethanol inhibited CuB-induced autophagy, as determined by MDC staining, autophagic protein expression, and transmission electron microscopy. Therefore, the present data suggested that ethanol enhanced CuB cytotoxicity in LO2 hepatocytes, which was mediated by inhibiting autophagy and augmenting apoptosis. |
Keyword | Apoptosis Autophagy Cucurbitacin b Ethanol Hepatocyte |
DOI | 10.1007/s13273-016-0005-2 |
URL | View the original |
Indexed By | SCI |
WOS Research Area | Biochemistry & Molecular Biology ; Toxicology |
WOS Subject | Biochemistry & Molecular Biology ; Toxicology |
WOS ID | WOS:000373095200005 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Institute of Chinese Medical Sciences |
Affiliation | 1.Guiyang College of Traditional Chinese Medicine 2.Zhejiang University 3.Universidade de Macau |
Recommended Citation GB/T 7714 | Ding Q.,Bao J.,Zhao W.,et al. Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes[J]. Molecular and Cellular Toxicology,2016,12(1):29-36. |
APA | Ding Q.,Bao J.,Zhao W.,Lu J.,Zhu H.,&Chen X..(2016).Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes.Molecular and Cellular Toxicology,12(1),29-36. |
MLA | Ding Q.,et al."Ethanol enhances cucurbitacin B-induced apoptosis by inhibiting cucurbitacin B-induced autophagy in LO2 hepatocytes".Molecular and Cellular Toxicology 12.1(2016):29-36. |
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