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Protein-directed DNA structure. I. Raman spectroscopy of a high- mobility-group box with application to human sex reversal
Benevides J.M.3; Chan G.2; Lu X.-J.1; Olson W.K.1; Weiss M.A.4; Thomas Jr. G.J.3
2000-01-25
Source PublicationBiochemistry
ISSN00062960
Volume39Issue:3Pages:537-547
Abstract

Protein-directed reorganization of DNA underlies mechanisms of transcription, replication, and recombination. A molecular model for DNA reorganization in the regulation of gene expression is provided by the sequence-specific high-mobility-group (HMG) box. Structures of HMG-box complexes with DNA are characterized by expansion of the minor groove, sharp bending toward the major groove, and local unwinding of the double helix. The Raman vibrational signature of such DNA reorganization has been identified in a study of the SRY HMG box, encoded by the human male-determining region of the Y chromosome. We observe in the human SRY-HMG:DNA complex extraordinarily large perturbations to Raman bands associated with vibrational modes of the DNA backbone and accompanying large increases in intensities of Raman bands attributable to base unstacking. In contrast, DNA major-groove binding, as occurs for the bZIP protein GCN4 [Benevides, J. M., Li, T., Lu, X.-J., Srinivasan, A. R., Olson, W. K., Weiss, M. A., and Thomas, G. J., Jr. (2000) Biochemistry 39, 548-556], perturbs the Raman signature of DNA only marginally. Raman markers of minor-groove recognition in the human SRY- HMG:DNA complex are due primarily to perturbation of specific vibrational modes of deoxyribose moieties and presumably reflect desolvation at the nonpolar interface of protein and DNA. These Raman markers may be diagnostic of protein-induced DNA bending and are proposed as a baseline for comparative analysis of mutations in SRY that cause human sex reversal.

DOI10.1021/bi9900525
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000084990800006
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Cited Times [WOS]:33   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWeiss M.A.
Affiliation1.Rutgers, The State University of New Jersey
2.University of Chicago
3.University of Missouri-Kansas City
4.CASE School of Medicine
Recommended Citation
GB/T 7714
Benevides J.M.,Chan G.,Lu X.-J.,et al. Protein-directed DNA structure. I. Raman spectroscopy of a high- mobility-group box with application to human sex reversal[J]. Biochemistry,2000,39(3):537-547.
APA Benevides J.M.,Chan G.,Lu X.-J.,Olson W.K.,Weiss M.A.,&Thomas Jr. G.J..(2000).Protein-directed DNA structure. I. Raman spectroscopy of a high- mobility-group box with application to human sex reversal.Biochemistry,39(3),537-547.
MLA Benevides J.M.,et al."Protein-directed DNA structure. I. Raman spectroscopy of a high- mobility-group box with application to human sex reversal".Biochemistry 39.3(2000):537-547.
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