UM
Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers
Zhu L.2; Wilken J.3; Phillips N.B.4; Narendra U.4; Chan G.2; Stratton S.M.3; Kent S.B.3; Weiss M.A.2
2000-07-15
Source PublicationGenes and Development
ISSN08909369
Volume14Issue:14Pages:1750-1764
AbstractSex determination is regulated by diverse pathways. Although upstream signals vary, a cysteine-rich DNA-binding domain (the DM motif) is conserved within downstream transcription factors of Drosophila melanogaster (Doublesex) and Caenorhabditis elegans (MAB-3). Vertebrate DM genes have likewise been identified and, remarkably, are associated with human sex reversal (46, XY gonadal dysgenesis). Here we demonstrate that the structure of the Doublesex domain contains a novel zinc module and disordered tail. The module consists of intertwined CCHC and HCCC Zn-binding sites; the tail functions as a nascent recognition α-helix. Mutations in either Zn- binding site or tail can lead to an intersex phenotype. The motif binds in the DNA minor groove without sharp DNA bending. These molecular features, unusual among zinc fingers and zinc modules, underlie the organization of a Drosophila enhancer that integrates sex- and tissue-specific signals. The structure provides a foundation for analysis of DM mutations affecting sexual dimorphism and courtship behavior.
KeywordDNA-binding protein Drosophila melanogaster Gene regulation NMR Transcription factor
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.City of Hope National Med Center
2.University of Chicago
3.Gryphon Therapeutics
4.CASE School of Medicine
Recommended Citation
GB/T 7714
Zhu L.,Wilken J.,Phillips N.B.,et al. Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers[J]. Genes and Development,2000,14(14):1750-1764.
APA Zhu L..,Wilken J..,Phillips N.B..,Narendra U..,Chan G..,...&Weiss M.A..(2000).Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers.Genes and Development,14(14),1750-1764.
MLA Zhu L.,et al."Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers".Genes and Development 14.14(2000):1750-1764.
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