UM  > 中華醫藥研究院
Determination of the levels of two types of neurotransmitter and the anti-migraine effects of different dose-ratios of Ligusticum chuanxiong and Gastrodia elata
Wang Q.1; Shen L.1; Ma S.-Y.1; Chen M.-W.2; Lin X.1; Hong Y.-L.1; Feng Y.1
2016
Source PublicationJournal of Food and Drug Analysis
ISSN10219498
Volume24Issue:1Pages:189-198
AbstractLigusticum chuanxiong (LC)-Gastrodia elata (GE) compatibility is widely used in the clinic for the treatment of migraine. It has been shown that the changes of neurotransmitters in the central nervous system are closely related to the pathogenesis of migraine; whether LC-GE compatibility might affect the neurotransmitters in migraine rats has not yet been studied. In this study, high performance liquid chromatography-fluorescence detector methods for quantification of serotonin (5-hydroxytryptamine, 5-HT) and excitatory amino acids (EAAs) in rat brain were developed. The 5-HT was measured directly, while EAAs were determined by using dansyl chloride as precolumn derivative reagent. The validation of the methods, including selectivity, linearity, sensitivity, precision, accuracy, recoveries, and stability were carried out and demonstrated to meet the requirements of quantitative analysis. Compared with the model group, the expression of 5-HT in migraine rat brain was enhanced from 30 minutes to 120 minutes and glutamate (L-Glu) was suppressed from 30 minutes to 60 minutes in an LC-GE (4:3) group compared with the model group (p < 0.05, p < 0.01, respectively). These findings showed that the analytical methods were simple, sensitive, selective, and low cost, and LC-GE 4:3 compatibility could have better efficacy for treating migraine through upregulating 5-HT levels and downregulating L-Glu levels.
Keywordexcitatory amino acids HPLC-FLD Ligusticum chuanxiong-Gastrodia elata compatibility migraine serotonin
DOI10.1016/j.jfda.2015.08.005
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Shanghai University of Traditional Chinese Medicine
2.Universidade de Macau
Recommended Citation
GB/T 7714
Wang Q.,Shen L.,Ma S.-Y.,et al. Determination of the levels of two types of neurotransmitter and the anti-migraine effects of different dose-ratios of Ligusticum chuanxiong and Gastrodia elata[J]. Journal of Food and Drug Analysis,2016,24(1):189-198.
APA Wang Q..,Shen L..,Ma S.-Y..,Chen M.-W..,Lin X..,...&Feng Y..(2016).Determination of the levels of two types of neurotransmitter and the anti-migraine effects of different dose-ratios of Ligusticum chuanxiong and Gastrodia elata.Journal of Food and Drug Analysis,24(1),189-198.
MLA Wang Q.,et al."Determination of the levels of two types of neurotransmitter and the anti-migraine effects of different dose-ratios of Ligusticum chuanxiong and Gastrodia elata".Journal of Food and Drug Analysis 24.1(2016):189-198.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang Q.]'s Articles
[Shen L.]'s Articles
[Ma S.-Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang Q.]'s Articles
[Shen L.]'s Articles
[Ma S.-Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang Q.]'s Articles
[Shen L.]'s Articles
[Ma S.-Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.