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Qualitative and quantitative characterization of phenolic and diterpenoid constituents in Danshen (Salvia miltiorrhiza) by comprehensive two-dimensional liquid chromatography coupled with hybrid linear ion trap Orbitrap mass
Cao J.-L.; Wei J.-C.; Hu Y.-J.; He C.-W.; Chen M.-W.; Wan J.-B.; Li P.
2016-01-04
Source PublicationJournal of Chromatography A
ISSN18733778 00219673
Volume1427Pages:79-89
Abstract

Danshen is one of the most frequently used traditional Chinese herbs owing to its remarkable and reliable therapeutic effects. Phenolic acids and diterpenoids have proved to be the bioactive substance groups. In order to fully profile its chemical compositions and explore new potential bioactive compounds, a comprehensive two-dimensional liquid chromatography system coupled to DAD detector and hybrid linear ion trap (LTQ) Orbitrap mass spectrometry (LC×LC-DAD-ESI/HRMS/MS) was set up in this study based on the column combination of Hypersil gold CN (150mm×1mm, 3μm) and Accucore C18 (50mm×4.6mm, 2.6μm). Using the optimal segment gradient program, phenolic acids and diterpenoids were separated into two independent groups and a total of 328 peaks were successfully detected on the contour plot of Danshen. By means of the accurate mass and reliable MS data, 102 compounds were identified or tentatively identified and 7 of them were discovered from Danshen for the first time. Moreover, the LC×LC-DAD system was validated for the quantitative analysis of 14 bioactive analytes using the contour plot, exhibiting satisfactory linearity (r≥0.9976) and high precision for both peak locating (≤1.07%) and peak volume calculating (0.34%-4.11%). The established method could afford powerful separation capability, reliable identification data and accurate quantitative results, which is very suitable for analysis of complex herbal samples.

KeywordComprehensive Two-dimensional Liquid Chromatography Danshen Diterpenoids Ltq Orbitrap Mass Spectrometry Phenolic Acids
DOI10.1016/j.chroma.2015.11.078
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000368969500010
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi P.
AffiliationUniversidade de Macau
Recommended Citation
GB/T 7714
Cao J.-L.,Wei J.-C.,Hu Y.-J.,et al. Qualitative and quantitative characterization of phenolic and diterpenoid constituents in Danshen (Salvia miltiorrhiza) by comprehensive two-dimensional liquid chromatography coupled with hybrid linear ion trap Orbitrap mass[J]. Journal of Chromatography A,2016,1427:79-89.
APA Cao J.-L..,Wei J.-C..,Hu Y.-J..,He C.-W..,Chen M.-W..,...&Li P..(2016).Qualitative and quantitative characterization of phenolic and diterpenoid constituents in Danshen (Salvia miltiorrhiza) by comprehensive two-dimensional liquid chromatography coupled with hybrid linear ion trap Orbitrap mass.Journal of Chromatography A,1427,79-89.
MLA Cao J.-L.,et al."Qualitative and quantitative characterization of phenolic and diterpenoid constituents in Danshen (Salvia miltiorrhiza) by comprehensive two-dimensional liquid chromatography coupled with hybrid linear ion trap Orbitrap mass".Journal of Chromatography A 1427(2016):79-89.
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