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A novel Nogo-66 receptor antagonist peptide promotes neurite regeneration in vitro
Sun Z.1; Dai X.1; Li Y.1; Jiang S.1; Lou G.1; Cao Q.1; Hu R.1; Huang Y.1; Su Z.1; Chen M.2; Luo H.1; Lin X.1; Sun J.1; Xiao F.1
2016-03-01
Source PublicationMolecular and Cellular Neuroscience
ISSN10959327 10447431
Volume71Pages:80-91
AbstractThe Nogo-66 receptor (NgR1), a receptor for Nogo-A, contributes to the inhibition of axonal regeneration in the adult central nervous system after traumatic injuries. Thus, NgR1 has been considered a critical target in axon regeneration therapy. Here, we identified a specific NgR1 antagonist peptide (HIYTALV, named NAP2) which promotes neurite regeneration in vitro from a phage display heptapeptide library. NAP2 was co-localized with NgR1 on the surface of PC12 cells and cerebellar granule cells (CGCs) by immunofluorescence assay. Horseradish peroxidase (HRP)-streptavidin-biotin assay further showed that NAP2 binds to NgR1 and the dissociation constant (Kd) was 0.45. μM Functional analyses indicated that NAP2 could reduce the inhibitory effects of Nogo-66 on neurite outgrowth in differentiated PC12 cells and CGCs by blocking the Nogo-66-induced activation of Rho-associated coiled coil-containing protein kinase (ROCK), collapsin response mediator protein 2 (CRMP2) and myosin light chain (MLC). Taken together, the small molecule NgR1 antagonist peptide NAP2 (MW: 815.98. Da) has a potential ability in crossing blood brain barrier and will be a promising therapeutic agent for the treatment of spinal cord injury and neurodegenerative diseases.
KeywordAntagonist peptide Neurite regeneration Nogo-66 receptor Phage display
DOI10.1016/j.mcn.2015.12.011
URLView the original
Language英語
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Jinan University
2.Universidade de Macau
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GB/T 7714
Sun Z.,Dai X.,Li Y.,et al. A novel Nogo-66 receptor antagonist peptide promotes neurite regeneration in vitro[J]. Molecular and Cellular Neuroscience,2016,71:80-91.
APA Sun Z..,Dai X..,Li Y..,Jiang S..,Lou G..,...&Xiao F..(2016).A novel Nogo-66 receptor antagonist peptide promotes neurite regeneration in vitro.Molecular and Cellular Neuroscience,71,80-91.
MLA Sun Z.,et al."A novel Nogo-66 receptor antagonist peptide promotes neurite regeneration in vitro".Molecular and Cellular Neuroscience 71(2016):80-91.
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