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Combination therapy of pDNA and siRNA by versatile carriers composed of poly(l-serine) modified polyethylenimines
Chen J.2; Guo Z.2; Lin L.2; Hu Y.2; Tian H.2; Chen M.1; Chen X.2
2017-05-01
Source PublicationMaterials Chemistry Frontiers
ISSN20521537
Volume1Issue:5Pages:937-946
AbstractMulti-gene-based approaches for tumor therapy have yet to be developed. Accordingly, we explored a combination treatment strategy by loading two kinds of therapeutic reagents simultaneously: pDNA and siRNA. Polyethylenimine-poly(l-serine) (PEI-PSer) obtained via ring-opening polymerization of l-serine N-carboxyanhydride initiated by polyethylenimines served as a potent cationic gene carrier. Proton nuclear magnetic resonance spectroscopy and gel permeation chromatography confirmed the successful preparation of PEI-PSer copolymers. The morphologies and effective binding capacities of PEI-PSer and genes were evaluated through gel retardation, scanning electron microscopy, and particle size and zeta potential assays. PEI-PSer displayed pDNA transfection and siRNA knockdown efficacy without affecting cell viability. The high gene transfer efficacy was mainly due to the excellent intracellular trafficking ability of PEI-PSer, as confirmed by confocal laser scanning microscopy and flow cytometry. Cell apoptosis assay further confirmed the combination antitumor effect aroused by the co-delivery of pKH-rev-casp-3 and siBcl2 by PEI-PSer. This study introduced a new carrier that delivers pDNA and siRNA simultaneously, thereby providing a potential strategy for tumor treatment.
DOI10.1039/c6qm00270f
URLView the original
Language英語
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Universidade de Macau
2.Changchun Institute of Applied Chemistry Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen J.,Guo Z.,Lin L.,et al. Combination therapy of pDNA and siRNA by versatile carriers composed of poly(l-serine) modified polyethylenimines[J]. Materials Chemistry Frontiers,2017,1(5):937-946.
APA Chen J..,Guo Z..,Lin L..,Hu Y..,Tian H..,...&Chen X..(2017).Combination therapy of pDNA and siRNA by versatile carriers composed of poly(l-serine) modified polyethylenimines.Materials Chemistry Frontiers,1(5),937-946.
MLA Chen J.,et al."Combination therapy of pDNA and siRNA by versatile carriers composed of poly(l-serine) modified polyethylenimines".Materials Chemistry Frontiers 1.5(2017):937-946.
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