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Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary
Li, Qiao-Qiao1; Li, Su-Ying2; Wang, Feng-Qin1; Chen, Hua1; Hu, Yuan-Jia3; Xia, Zhi-Ning1; Yang, Feng-Qing1
2018-03
Source PublicationCHROMATOGRAPHIA
ISSN0009-5893
Volume81Issue:3Pages:509-516
Abstract

Capillary electrophoresis (CE) has been widely used in the study of the interactions between targets (biological macromolecules) and ligands due to its good biocompatibility. However, the separation and analysis of platelets, which play a pivotal role in thrombotic diseases, were limited owing to platelets adhesion in inner wall of capillary during CE analysis. In this paper, polyvinyl alcohol-coated capillaries were simply prepared to prevent the adhesion of platelet. Then, a frontal analysis CE (FACE) method was developed to evaluate the interactions between platelets and eight alkaloids include glaucine, magnoflorine, dehydrocorydaline, palmatine, berberine, isorhynchophylline, rhynchophylline, and brucine. The binding constants and stoichiometries of the interactions were calculated by Scatchard equation for strong and specific interaction, and the non-specific binding equation for weak interaction. The results indicated that glaucine, dehydrocorydaline, and isorhynchophylline showed relatively high affinity interaction with platelets. The developed FACE method may be further applied in the evaluation of interactions between platelets and other small molecular compounds.

KeywordFrontal Analysis Capillary Electrophoresis Platelets Interaction Alkaloids Polyvinyl Alcohol
DOI10.1007/s10337-018-3476-6
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000426858600013
PublisherSPRINGER HEIDELBERG
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYang, Feng-Qing
Affiliation1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, People’s Republic of China
2.Department of Pharmacy, Henan Medical College, Zhengzhou 451191, People’s Republic of China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, People’s Republic of China
Recommended Citation
GB/T 7714
Li, Qiao-Qiao,Li, Su-Ying,Wang, Feng-Qin,et al. Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary[J]. CHROMATOGRAPHIA,2018,81(3):509-516.
APA Li, Qiao-Qiao.,Li, Su-Ying.,Wang, Feng-Qin.,Chen, Hua.,Hu, Yuan-Jia.,...&Yang, Feng-Qing.(2018).Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary.CHROMATOGRAPHIA,81(3),509-516.
MLA Li, Qiao-Qiao,et al."Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary".CHROMATOGRAPHIA 81.3(2018):509-516.
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