Please use this identifier to cite or link to this item: http://repository.umac.mo/handle/10692/8284
Title: Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by a coculture of Pseudomonas putida and Pseudomonas fluorescens immobilized in a fibrous-bed bioreactor
Authors: Shim, Hojae
Yang, Shang-Tian
Issue Date: 11-Sep-1998
Publisher: Elsevier
Citation: Journal of Biotechnology. Jan 1999, Vol 67, Issue 2-3, p.99-112
Abstract: A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-bed bioreactor and free cells grown in serum bottles were studied. In general, the BTEX biodegradation rate increased with increasing substrate concentration and then decreased after reaching a maximum, showing substrate-inhibition kinetics. However, for immobilized cells, the degradation rate was much higher than that of free cells. Compared to free cells, immobilized cells in the bioreactor tolerated higher concentrations (>1000 mg l−1) of benzene and toluene, and gave at least 16-fold higher degradation rates for benzene, ethylbenzene, and o-xylene, and a 9-fold higher degradation rate for toluene. Complete and simultaneous degradation of BTEX mixture was achieved in the bioreactor under hypoxic conditions. Cells in the bioreactor were relatively insensitive to benzene toxicity; this insensitivity was attributed to adaptation of the cells in the bioreactor. Compared to the original seeding culture, the adapted cells from the fibrous-bed bioreactor had higher specific growth rate, benzene degradation rate, and cell yield when the benzene concentration was higher than 100 mg l−1. Cells in the fibrous bed had a long, slim morphology, which is different from the normal short-rod shape found for suspended cells in solution.
URI: http://repository.umac.mo/handle/10692/8284
ISSN: 0168-1656
1873-4863
Keywords: BTEX
Biodegradation
Fibrous-bed bioreactor
Pseudomonas putida
Pseudomonas fluorescens
Access: https://doi.org/10.1016/S0168-1656(98)00166-7

Files in This Item:
File Description SizeFormat 
26817_0_JBiotech-1999.pdf1.09 MBAdobe PDFView/Open
Appears in Collections:CEE Journal Articles




Items in UMIR are protected by copyright, with all rights reserved, unless otherwise indicated.