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An ortho-aldehyde modified probe to image thiols in living cells with enhanced selectivity
Xing, Panfei1; Shi, Yuchen1; Li, Qiu1; Feng, Yanxian1; Dong, Lei2; Wang, Chunming1
2018-03
Source PublicationTALANTA
ISSN0039-9140
Volume179Pages:326-330
Abstract

Developing fluorescent probes to image thiols in the living system may provide powerful tools to study the functions of thiol-containing biological molecules. In this study, we report the design and evaluation of a novel turn-on fluorescent probe NQNO for selective detection of thiols in living cells. By introducing an ortho-aldehyde group to NNO, a conventional compound representing a class of thiol-imaging strategy, we obtained NQNO with enhanced selectivity for thiols over the major interferent hydrogen sulfide (H2S). NQNO could be applied in phosphate-buffered saline (PBS), where the efficacy of NNO was usually weakened. Notably, NQNO demonstrated solid performance in imaging endogenous thiols in living cells without exerting cytotoxicity. In summary, NQNO has the potential to serve as a safe, sensitive and effective fluorescent probe for thiol imaging in biological systems.

KeywordOrtho-aldehyde Fluorescent Probe Thiols Hydrogen Sulfide Bioimaging
DOI10.1016/j.talanta.2017.11.035
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000424178000043
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Co-First AuthorXing, Panfei
Corresponding AuthorWang, Chunming
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Macau SAR, China
2.State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Xing, Panfei,Shi, Yuchen,Li, Qiu,et al. An ortho-aldehyde modified probe to image thiols in living cells with enhanced selectivity[J]. TALANTA,2018,179:326-330.
APA Xing, Panfei,Shi, Yuchen,Li, Qiu,Feng, Yanxian,Dong, Lei,&Wang, Chunming.(2018).An ortho-aldehyde modified probe to image thiols in living cells with enhanced selectivity.TALANTA,179,326-330.
MLA Xing, Panfei,et al."An ortho-aldehyde modified probe to image thiols in living cells with enhanced selectivity".TALANTA 179(2018):326-330.
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