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SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins
Li, Chun-Hong; Zuo, Hua-Li; Chen, Cen; Hu, Yuan-Jia; Qian, Zheng-Ming; Li, Wen-Jia; Xia, Zhi-Ning; Yang, Feng-Qing
2018-03
Source PublicationPAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
ISSN1011-601X
Volume31Issue:2Pages:447-454
AbstractGanoderma lucidum (Chizhi in Chinese) is one of the most valuable and widely used medicinal fungi in traditional Chinese medicines (TCMs). Most of previous studies were focused on the triterpenoids and polysaccharides of G lucidum, whereas less attention had been paid on the protein, which is another bioactive compound in it. In the present study, protein maps of fourteen G lucidum samples were comprehensively analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). The results indicated that there were significant differences in protein profiles of G lucidum samples from different origins. Furthermore, previous reported bioactive proteins from the fruiting bodies of G lucidum, were mainly distributed in 4 taxa (A, B, C and D) based on their molecular weights on the 2-DE maps. The proteins should be considered as marker for the quality control of G lucidum, because the proteomic variation may affect on their pharmacological activities.
KeywordGanoderma lucidum SDS-PAGE 2-DE bioactive protein protein profiles
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000428228300013
PublisherUNIV KARACHI
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Li, Chun-Hong,Zuo, Hua-Li,Chen, Cen,et al. SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins[J]. PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,2018,31(2):447-454.
APA Li, Chun-Hong.,Zuo, Hua-Li.,Chen, Cen.,Hu, Yuan-Jia.,Qian, Zheng-Ming.,...&Yang, Feng-Qing.(2018).SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins.PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,31(2),447-454.
MLA Li, Chun-Hong,et al."SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins".PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 31.2(2018):447-454.
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