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IGF-1-Mediated Survival from Induced Death of Human Primary Cultured Retinal Pigment Epithelial Cells Is Mediated by an Akt-Dependent Signaling Pathway
Zheng, Wenhua1,2; Meng, Qian1; Wang, Haitao1,3; Yan, Fengxia1; Little, Peter J.4; Deng, Xinguo2; Lin, Shaofen2
2018-03
Source PublicationMOLECULAR NEUROBIOLOGY
ISSN0893-7648
Volume55Issue:3Pages:1915-1927
Abstract

Degeneration of the human retinal pigmented epithelium (hRPE) is involved in several eye disorders such as age-related macular degeneration (AMD). In this study, we investigated the protective effect of IGF-1 on human primary cultured RPE cells and its underlying mechanism. IGF-1 dose- and time-dependently promoted the survival of RPE cells from serum deprivation. Western blot showed that IGF-1 stimulated the activation of the PI3K/Akt and MAPK pathways in hRPE. Inhibition of the PI3K/Akt pathway by the PI3K-specific inhibitor, LY294002 or inhibition of Akt by Akt-specific inhibitors Akt inhibitor VIII or SN-38, or downregulation Akt with siRNA specific for Akt blocked the effect of IGF-1 on hRPE. In contrast, blockade of the MAPK pathway with a specific inhibitor PD98059 had no effect. Interestingly, vitreous IGF-1 injection reversed the inhibitory effect of light exposure (a dry AMD model) on both a wave and b wave. Immunocytochemistry showed that vitreous IGF-1 injections promoted the survival of RPE cells in rat retina and the expression of RPE65 in RPE cells from light injury. These results indicate that IGF-1 is able to protect hRPE cell from different insults in vivo and in vitro. Further detailed studies may lead the way to a therapeutic intervention for retinal diseases in which cell death is an underlying contributory mechanism.

KeywordRetinopathy Serum Deprivation Cell Signaling Apoptosis Growth Factors
DOI10.1007/s12035-017-0447-0
URLView the original
Indexed BySCI
Language英语
WOS Research AreaNeurosciences & Neurology
WOS SubjectNeurosciences
WOS IDWOS:000426897800008
PublisherHUMANA PRESS INC
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Faculty of Health Sciences, University of Macau, MacauChina
2.State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center and School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China
3.School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
4.School of Pharmacy, Pharmacy Australia Centre of Excellence (PACE), The University of Queensland, Woolloongabba, Australia
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Zheng, Wenhua,Meng, Qian,Wang, Haitao,et al. IGF-1-Mediated Survival from Induced Death of Human Primary Cultured Retinal Pigment Epithelial Cells Is Mediated by an Akt-Dependent Signaling Pathway[J]. MOLECULAR NEUROBIOLOGY,2018,55(3):1915-1927.
APA Zheng, Wenhua.,Meng, Qian.,Wang, Haitao.,Yan, Fengxia.,Little, Peter J..,...&Lin, Shaofen.(2018).IGF-1-Mediated Survival from Induced Death of Human Primary Cultured Retinal Pigment Epithelial Cells Is Mediated by an Akt-Dependent Signaling Pathway.MOLECULAR NEUROBIOLOGY,55(3),1915-1927.
MLA Zheng, Wenhua,et al."IGF-1-Mediated Survival from Induced Death of Human Primary Cultured Retinal Pigment Epithelial Cells Is Mediated by an Akt-Dependent Signaling Pathway".MOLECULAR NEUROBIOLOGY 55.3(2018):1915-1927.
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