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AMPK is involved in mediation of erythropoietin influence on metabolic activity and reactive oxygen species production in white adipocytes
Wang L.1; Di L.1; Noguchi C.T.2
2014
Source PublicationInternational Journal of Biochemistry and Cell Biology
ISSN18785875 13572725
Volume54Pages:1-9
Abstract

Erythropoietin, discovered for its indispensable role during erythropoiesis, has been used in therapy for selected red blood cell disorders in erythropoietin-deficient patients. The biological activities of erythropoietin have been found in animal models to extend to non-erythroid tissues due to the expression of erythropoietin receptor. We previously demonstrated that erythropoietin promotes metabolic activity and white adipocytes browning to increase mitochondrial function and energy expenditure via peroxisome proliferator-activated receptor alpha and Sirtuin1. Here we report that AMP-activated protein kinase was activated by erythropoietin possibly via Ca/calmodulin-dependent protein kinase kinase in adipocytes as well as in white adipose tissue from diet induced obese mice. Erythropoietin increased cellular nicotinamide adenine dinucleotide via increased AMP-activated protein kinase activity, possibly leading to Sirtuin1 activation. AMP-activated protein kinase knock down reduced erythropoietin mediated increase in cellular oxidative function including the increased oxygen consumption rate, fatty acid utilization and induction of key metabolic genes. Under hypoxia, adipocytes were found to generate more reactive oxygen species, and erythropoietin reduced the reactive oxygen species and increased antioxidant gene expression, suggesting that erythropoietin may provide protection from oxidative stress in adipocytes. Erythropoietin also reversed increased nicotinamide adenine dinucleotide by hypoxia via increased AMP-activated protein kinase. Additionally, AMP-activated protein kinase is found to be involved in erythropoietin stimulated increase in oxygen consumption rate, fatty acid oxidation and mitochondrial gene expression. AMP-activated protein kinase knock down impaired erythropoietin stimulated increases in antioxidant gene expression. Collectively, our findings identify the AMP-activated protein kinase involvement in erythropoietin signaling in regulating adipocyte cellular redox status and metabolic activity. © 2014 Elsevier Ltd.

KeywordAdipocytes Amp-activated Protein Kinase Erythropoietin Oxidative Metabolism Reactive Oxygen Species
DOI10.1016/j.biocel.2014.06.008
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000342524600001
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Cited Times [WOS]:182   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Universidade de Macau
2.National Institute of Diabetes and Digestive and Kidney Diseases
Recommended Citation
GB/T 7714
Wang L.,Di L.,Noguchi C.T.. AMPK is involved in mediation of erythropoietin influence on metabolic activity and reactive oxygen species production in white adipocytes[J]. International Journal of Biochemistry and Cell Biology,2014,54:1-9.
APA Wang L.,Di L.,&Noguchi C.T..(2014).AMPK is involved in mediation of erythropoietin influence on metabolic activity and reactive oxygen species production in white adipocytes.International Journal of Biochemistry and Cell Biology,54,1-9.
MLA Wang L.,et al."AMPK is involved in mediation of erythropoietin influence on metabolic activity and reactive oxygen species production in white adipocytes".International Journal of Biochemistry and Cell Biology 54(2014):1-9.
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