UM
BioID: A proximity-dependent labeling approach in proteomics study
Li P.; Meng Y.; Wang L.; Di L.-J.
2019
AbstractBiological activities are mainly executed by proteins and in most of the occasions these activities are accomplished by protein complexes or through protein–protein interactions (PPI). So it is critical to reveal how the protein complexes are organized and demonstrate the PPIs involved in the biological processes. In addition to the traditional biochemical approaches, proximity-dependent labeling (PDL) has recently been proposed to identify the interacting partners of a given protein. PDL requires the fusion expression of the target protein with an enzyme which catalyzes the attachment of a reactive molecule to the interacting partners in a distance-dependent manner. Further analysis of all the proteins that are modified by the reactive molecule discloses the identity of these proteins which are presumed to be interacting partners of the target protein. BioID is one of those representative PDL methods with the most widely applications. The enzyme used in BioID is a biotin ligase BirA which catalyzes the biotinylation of target protein with the presence of biotin. Through streptavidin-mediated pull-down and mass spectrometry analysis, the interacting protein candidates of a given protein can be obtained.
KeywordBioID Protein–protein interactions Proximity-dependent labeling
DOI10.1007/978-1-4939-8814-3_10
URLView the original
Pages143-151
Language英語
Fulltext Access
Citation statistics
Document TypeBook
CollectionUniversity of Macau
AffiliationUniversidade de Macau
Recommended Citation
GB/T 7714
Li P.,Meng Y.,Wang L.,et al. BioID: A proximity-dependent labeling approach in proteomics study[M],2019.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li P.]'s Articles
[Meng Y.]'s Articles
[Wang L.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li P.]'s Articles
[Meng Y.]'s Articles
[Wang L.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li P.]'s Articles
[Meng Y.]'s Articles
[Wang L.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.