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Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells
Chen G.1; Gulbranson D.R.1; Yu P.1; Hou Z.1; Thomson J.A.1
2012-04-01
Source PublicationStem Cells
ISSN10665099
Volume30Issue:4Pages:623-630
Abstract

Fibroblast growth factor (FGF), transforming growth factor (TGF)/Nodal, and Insulin/insulin-like growth factor (IGF) signaling pathways are sufficient to maintain human embryonic stem cells (ESCs) and induced pluripotent stem cells in a proliferative, undifferentiated state. Here, we show that only a few FGF family members (FGF2, FGF4, FGF6, and FGF9) are able to sustain strong extracellular-signal-regulated kinase (ERK) phosphorylation and NANOG expression levels in human ESCs. Surprisingly, FGF1, which is reported to target the same set of receptors as FGF2, fails to sustain ERK phosphorylation and NANOG expression under standard culture conditions. We find that the failure of FGF1 to sustain ES is due to thermal instability of the wild-type protein, not receptor specificity, and that a mutated thermal- stable FGF1 sustains human ESCs and supports both differentiation and reprogramming protocols. © AlphaMed Press.

KeywordDifferentiation Fibroblast Growth Factor Map Kinase Pluripotency Pluripotent Stem Cells Thermal Stability
DOIhttp://doi.org/10.1002/stem.1021
URLView the original
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
WOS SubjectCell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
WOS IDWOS:000302005000006
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Cited Times [WOS]:59   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Morgridge Institute for Research
2.University of California, Santa Barbara
3.University of Wisconsin Madison
4.University of Wisconsin School of Medicine and Public Health
Recommended Citation
GB/T 7714
Chen G.,Gulbranson D.R.,Yu P.,et al. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells[J]. Stem Cells,2012,30(4):623-630.
APA Chen G.,Gulbranson D.R.,Yu P.,Hou Z.,&Thomson J.A..(2012).Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells.Stem Cells,30(4),623-630.
MLA Chen G.,et al."Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells".Stem Cells 30.4(2012):623-630.
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