UM  > 健康科學學院
Heparin promotes cardiac differentiation of human pluripotent stem cells in chemically defined albumin-free medium, enabling consistent manufacture of cardiomyocytes
Lin Y.2; Linask K.L.2; Mallon B.1; Johnson K.5; Klein M.3; Beers J.2; Xie W.2; Du Y.2; Liu C.2; Lai Y.6; Zou J.2; Haigney M.3; Yang H.6; Rao M.1; Chen G.2
2017-02-01
Source PublicationStem Cells Translational Medicine
ISSN21576580 21576564
Volume6Issue:2Pages:527-538
Abstract

Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate hPSCs into cardiomyocytes. The treatment with heparin helped cardiomyocyte differentiation consistently reach at least 80% purity (up to 95%) from more than 10 different hPSC lines in chemically defined Dulbecco’s modified Eagle’s medium/F-12-based medium on either Matrigel or defined matrices like vitronectin and Synthemax. One of heparin’s main functions was to act as a Wnt modulator that helped promote robust and consistent cardiomyocyte production. Our study provides an efficient, reliable, and cost-effective method for cardiomyocyte derivation from hPSCs that can be used for potential large-scale drug screening, disease modeling, and future cellular therapies.

KeywordCardiac Cell Culture Embryonic Stem Cells Heparin Induced Pluripotent Stem Cells
DOI10.5966/sctm.2015-0428
URLView the original
Indexed BySCI
Language英语
WOS Research AreaCell Biology
WOS SubjectCell & Tissue Engineering
WOS IDWOS:000398198500018
Fulltext Access
Citation statistics
Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.National Institutes of Health, Bethesda
2.National Heart, Lung, and Blood Institute
3.Uniformed Services University of the Health Sciences
4.Universidade de Macau
5.National Institute of Neurological Disorders and Stroke
6.Thomas Jefferson University
Recommended Citation
GB/T 7714
Lin Y.,Linask K.L.,Mallon B.,et al. Heparin promotes cardiac differentiation of human pluripotent stem cells in chemically defined albumin-free medium, enabling consistent manufacture of cardiomyocytes[J]. Stem Cells Translational Medicine,2017,6(2):527-538.
APA Lin Y..,Linask K.L..,Mallon B..,Johnson K..,Klein M..,...&Chen G..(2017).Heparin promotes cardiac differentiation of human pluripotent stem cells in chemically defined albumin-free medium, enabling consistent manufacture of cardiomyocytes.Stem Cells Translational Medicine,6(2),527-538.
MLA Lin Y.,et al."Heparin promotes cardiac differentiation of human pluripotent stem cells in chemically defined albumin-free medium, enabling consistent manufacture of cardiomyocytes".Stem Cells Translational Medicine 6.2(2017):527-538.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lin Y.]'s Articles
[Linask K.L.]'s Articles
[Mallon B.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lin Y.]'s Articles
[Linask K.L.]'s Articles
[Mallon B.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lin Y.]'s Articles
[Linask K.L.]'s Articles
[Mallon B.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.