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A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals
Saragovi H.U.1; Zheng W.1; Maliartchouk S.1; DiGugliemo G.M.1; Mawal Y.R.1; Kamen A.4; Woo S.B.3; Cuello A.C.1; Debeir T.1; Neet K.E.3
1998-12-25
Source PublicationJournal of Biological Chemistry
ISSN00219258
Volume273Issue:52Pages:34933-34940
AbstractNerve growth factor (NGF) is a neurotrophin that induces neuritogenic and trophic signals by binding to TrkA and/or p75 receptors. We report a comparative study of the binding, internalization, and biological activity of NGF versus that of NGF in association with an anti-NGF monoclonal antibody (mAb NGF30), directed against the C termini of NGF. NGF·mAb complexes do not bind p75 effectively but bind TrkA with high affinity. After binding, NGF·mAb complexes stimulate internalization faster and to a larger degree than NGF. NGF·mAb-induced activation of TrkA, Shc, and MAPK is transient compared with NGF-induced activation; yet NGF and NGF·mAb afford identical trophic responses. In contrast, NGF induces Suc-1-associated neurotrophic activating protein phosphorylation and neuritogenic differentiation, but NGF·mAb does not. Thus, an absolute separation of trophic and neuritogenic function is seen for NGF·mAb, suggesting that biological response modifiers of neurotrophins can afford ligands with selected activities.
DOI10.1074/jbc.273.52.34933
URLView the original
Language英語
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Cited Times [WOS]:79   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.McGill University
2.McGill University, Rosalind and Morris Goodman Cancer Research Centre
3.Chicago Medical School
4.National Research Council Canada
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GB/T 7714
Saragovi H.U.,Zheng W.,Maliartchouk S.,et al. A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals[J]. Journal of Biological Chemistry,1998,273(52):34933-34940.
APA Saragovi H.U..,Zheng W..,Maliartchouk S..,DiGugliemo G.M..,Mawal Y.R..,...&Neet K.E..(1998).A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals.Journal of Biological Chemistry,273(52),34933-34940.
MLA Saragovi H.U.,et al."A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals".Journal of Biological Chemistry 273.52(1998):34933-34940.
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