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TGF-β stimulates biglycan core protein synthesis but not glycosaminoglycan chain elongation via Akt phosphorylation in vascular smooth muscle
Osman N.3; Getachew R.3; Burch M.3; Lancaster G.1; Wang R.2; Wang H.2; Zheng W.2; Little P.J.3
2011-10-01
Source PublicationGrowth Factors
ISSN08977194 10292292
Volume29Issue:5Pages:203-210
AbstractTransforming growth factor-β (TGF-β) can mediate proteoglycan synthesis via Smad and non-Smad signalling pathways in vascular smooth muscle (VSM). We investigated whether TGF-β-mediated proteoglycan synthesis is via PI3K/Akt. TGF-β induced a rapid phosphorylation of Akt that continued upto 4 h. Akt phosphorylation was blocked by Akt1/2 inhibitor SN30978; however, it did not block Smad2 phosphorylation at either the carboxy or linker regions indicating that TGF-β-mediated Akt phosphorylation is independent of Smad2 signalling. The role of Akt in TGF-β-mediated proteoglycan synthesis was investigated. Treatment with SN30978 showed a concentration-dependent decrease in TGF-β-mediated [ S]-sulphate and [ S]-Met/Cys incorporation into secreted proteoglycans; however, SDS-PAGE showed no change in biglycan size. In TGF-β-treated cells, biglycan mRNA levels increased by 40100% in 24 h and was significantly blocked by SN30978. Our findings demonstrate that Akt is a downstream signalling component of TGF-β-mediated biglycan core protein synthesis but not glycosaminoglycan chain hyper-elongation in VSM. © 2011 Informa UK, Ltd.
KeywordAkt Atherosclerosis Biglycan Transforming growth factor-β
DOI10.3109/08977194.2011.615747
URLView the original
Language英語
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Baker Heart and Diabetes Institute
2.Sun Yat-Sen University
3.RMIT University
Recommended Citation
GB/T 7714
Osman N.,Getachew R.,Burch M.,et al. TGF-β stimulates biglycan core protein synthesis but not glycosaminoglycan chain elongation via Akt phosphorylation in vascular smooth muscle[J]. Growth Factors,2011,29(5):203-210.
APA Osman N..,Getachew R..,Burch M..,Lancaster G..,Wang R..,...&Little P.J..(2011).TGF-β stimulates biglycan core protein synthesis but not glycosaminoglycan chain elongation via Akt phosphorylation in vascular smooth muscle.Growth Factors,29(5),203-210.
MLA Osman N.,et al."TGF-β stimulates biglycan core protein synthesis but not glycosaminoglycan chain elongation via Akt phosphorylation in vascular smooth muscle".Growth Factors 29.5(2011):203-210.
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