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(S)-[6]-Gingerol inhibits TGF-β-stimulated biglycan synthesis but not glycosaminoglycan hyperelongation in human vascular smooth muscle cells
Kamato D.5; Babaahmadi Rezaei H.5; Getachew R.5; Thach L.5; Guidone D.5; Osman N.5; Roufogalis B.2; Duke C.C.2; Tran V.H.2; Zheng W.4; Little P.J.5
2013-07-01
Source PublicationJournal of Pharmacy and Pharmacology
ISSN00223573 20427158
Volume65Issue:7Pages:1026-1036
AbstractObjectives: (S)-[6]-Gingerol is under investigation for a variety of therapeutic uses. Transforming growth factor (TGF)-β stimulates proteoglycan synthesis, leading to increased binding of low-density lipoproteins, which is the initiating step in atherosclerosis. We evaluated the effects of (S)-[6]-gingerol on these TGF-β-mediated proteoglycan changes to explore its potential as an anti-atherosclerotic agent. Methods: Purified (S)-[6]-gingerol was assessed for its effects on proteoglycan synthesis by [S]-sulfate incorporation into glycosaminoglycan chains and [ S]-Met/Cys incorporation into proteoglycans and total proteins in human vascular smooth muscle cells. Biglycan level was assessed by real-time quantitative polymerase chain reactions and the effects of (S)-[6]-gingerol on TGF-β signalling by assessment of the phosphorylation of Smads and Akt by western blotting. Key findings: (S)-[6]-Gingerol concentration-dependently inhibited TGF-β-stimulated proteoglycan core protein synthesis, and this was not secondary to inhibition of total protein synthesis. (S)-[6]-Gingerol inhibited biglycan mRNA expression. (S)-[6]-Gingerol did not inhibit TGF-β-stimulated glycosaminoglycan hyperelongation or phosphorylation of Smad 2, in either the carboxy terminal or linker region, or Akt phosphorylation. Conclusions: The activity of (S)-[6]-gingerol to inhibit TGF-β-stimulated biglycan synthesis suggests a potential role for ginger in the prevention of atherosclerosis or other lipid-binding diseases. The signalling studies indicate a novel site of action of (S)-[6]-gingerol in inhibiting TGF-β responses. © 2013 Royal Pharmaceutical Society.
Keyword(S)-[6]-Gingerol biglycan proteoglycans Smad signalling
DOI10.1111/jphp.12060
URLView the original
Language英語
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Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Monash University
2.The University of Sydney
3.Ahvaz Jundishapur University of Medical Sciences
4.Sun Yat-Sen University
5.RMIT University
Recommended Citation
GB/T 7714
Kamato D.,Babaahmadi Rezaei H.,Getachew R.,et al. (S)-[6]-Gingerol inhibits TGF-β-stimulated biglycan synthesis but not glycosaminoglycan hyperelongation in human vascular smooth muscle cells[J]. Journal of Pharmacy and Pharmacology,2013,65(7):1026-1036.
APA Kamato D..,Babaahmadi Rezaei H..,Getachew R..,Thach L..,Guidone D..,...&Little P.J..(2013).(S)-[6]-Gingerol inhibits TGF-β-stimulated biglycan synthesis but not glycosaminoglycan hyperelongation in human vascular smooth muscle cells.Journal of Pharmacy and Pharmacology,65(7),1026-1036.
MLA Kamato D.,et al."(S)-[6]-Gingerol inhibits TGF-β-stimulated biglycan synthesis but not glycosaminoglycan hyperelongation in human vascular smooth muscle cells".Journal of Pharmacy and Pharmacology 65.7(2013):1026-1036.
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