UM
Gardenamide A attenuated cell apoptosis induced by serum deprivation insult via the ERK1/2 and PI3K/AKT signaling pathways
Wang R.4; Yang J.6; Peng L.6; Zhao J.6; Mu N.2; Huang J.3; Lazarovici P.5; Chen H.6; Zheng W.4
2015-02-02
Source PublicationNeuroscience
ISSN18737544 03064522
Volume286Pages:242-250
AbstractGardenamide A (GA) is a stable genipin derivative with neuroprotective properties. It rescued pheochromocytoma cell (PC12) sympathetic cultures and retinal neuronal cells from apoptosis insult induced by serum deprivation. GA attenuated the accumulation of intracellular reactive oxygen species (ROS) and the loss of mitochondrial membrane potential. Western blotting with specific phospho-antibodies indicated that GA increased the phosphorylation of both the protein kinase B (Akt) and the extracellular signal-regulated kinase (ERK1/2) in PC12 cells. The GA neuroprotective effect was inhibited by either the specific phosphoinositide 3-kinase (PI3K) inhibitor LY294002 or the mitogen-activated protein kinase (MAPK) pathway inhibitor PD98059. These results propose that the neuroprotective effect of GA on PC12 neuronal cell cultures was mediated through both the PI3K/Akt and ERK1/2 signaling pathways. Therefore, GA may serve as a pharmacological tool to investigate neuroprotective mechanisms of neurons afflicted by different insults.
KeywordAkt Cell apoptosis ERK1/2 Gardenamide A ROS Serum deprivation
DOI10.1016/j.neuroscience.2014.11.056
URLView the original
Language英語
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Cited Times [WOS]:26   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research
2.Guangzhou Brain Hospital
3.Family Planning Service Center of Dongguan
4.Zhongshan Ophthalmic Center
5.Hebrew University of Jerusalem
6.Jinan University
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GB/T 7714
Wang R.,Yang J.,Peng L.,et al. Gardenamide A attenuated cell apoptosis induced by serum deprivation insult via the ERK1/2 and PI3K/AKT signaling pathways[J]. Neuroscience,2015,286:242-250.
APA Wang R..,Yang J..,Peng L..,Zhao J..,Mu N..,...&Zheng W..(2015).Gardenamide A attenuated cell apoptosis induced by serum deprivation insult via the ERK1/2 and PI3K/AKT signaling pathways.Neuroscience,286,242-250.
MLA Wang R.,et al."Gardenamide A attenuated cell apoptosis induced by serum deprivation insult via the ERK1/2 and PI3K/AKT signaling pathways".Neuroscience 286(2015):242-250.
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