UM  > 中華醫藥研究院
Artemisinin protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of ERK/CREB signaling
Chong C.-M.; Zheng W.
2016-10-01
Source PublicationRedox Biology
ISSN22132317
Volume9Pages:50-56
Abstract

The pathological increase in the levels of reactive oxygen species (ROS) in the retinal pigment epithelium (RPE), is implicated in the development of age-related macular degeneration (AMD). The discovery of drug candidates to effectively protect RPE cells from oxidative damage is required to resolve the pathological aspects and modify the process of AMD. In this study, a FDA-approved anti-malaria drug, Artemisinin was found to suppress hydrogen peroxide (HO)-induced cell death in human RPE cell-D407 cells. Further study showed that Artemisinin significantly suppressed HO induced D407 cell death by restoring abnormal changes in nuclear morphology, intracellular ROS, mitochondrial membrane potential and apoptotic biomarkers. Western blotting analysis showed that Artemisinin was able to activate extracellular regulated ERK/CREB survival signaling. Furthermore, Artemisinin failed to suppress HO-induced cytotoxicity and the increase of caspase 3/7 activity in the presence of the ERK inhibitor PD98059. Taken together, these results suggest that Artemisinin is a potential protectant with the pro-survival effects against HO insult through activation of the ERK/CREB pathway.

KeywordAge-related Macular Degeneration Artemisinin Erk Retinal Pigment Epithelial Cells Ros
DOI10.1016/j.redox.2016.06.002
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000386757000007
Fulltext Access
Citation statistics
Cited Times [WOS]:27   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Faculty of Health Sciences
AffiliationUniversidade de Macau
Recommended Citation
GB/T 7714
Chong C.-M.,Zheng W.. Artemisinin protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of ERK/CREB signaling[J]. Redox Biology,2016,9:50-56.
APA Chong C.-M.,&Zheng W..(2016).Artemisinin protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of ERK/CREB signaling.Redox Biology,9,50-56.
MLA Chong C.-M.,et al."Artemisinin protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of ERK/CREB signaling".Redox Biology 9(2016):50-56.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chong C.-M.]'s Articles
[Zheng W.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chong C.-M.]'s Articles
[Zheng W.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chong C.-M.]'s Articles
[Zheng W.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.