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Berberine protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of AMPK
Li S.3; Gaur U.3; Chong C.-M.3; Lin S.2; Fang J.3; Zeng Z.3; Wang H.4; Zheng W.3
2018-06-12
Source PublicationInternational Journal of Molecular Sciences
ISSN14220067 16616596
Volume19Issue:6
AbstractAge-related macular degeneration (AMD) is the leading cause of central vision loss in the elderly with less effective treatment, especially for dry AMD (90% of AMD). Although the etiology of this disease is not well elucidated, increasing evidences indicate that excessive reactive oxygen species (ROS) impairing the physiological functions of retinal pigment epithelium (RPE) cells may be one of the main causes. Therefore, it could be a great strategy to find some drugs that can effectively protect RPE cells from oxidative damage which is desired to treat and slow the process of AMD. In the present study, a well-known traditional Chinese medicine berberine (BBR) was found to suppress hydrogen peroxide (H O)-induced oxidative damage in D407 cells, a human RPE cell line. Pre-treatment of D407 cells with BBR significantly suppressed H O-induced cell apoptosis by restoring abnormal changes in nuclear morphology, preventing the decline of mitochondrial membrane potential, reducing lactate dehydrogenase release and inhibiting caspase 3/7 activities induced by H O. Western blot analysis showed that BBR was able to stimulate the phosphorylation/activation of AMPK in a time-and dose-dependent manner in D407 cells, while treatment of cells with AMPK pathway inhibitor Compound C, or knockdown of the AMPK by specific siRNA blocked the effect of BBR. Similar results were obtained in primary cultured human RPE cells. Taken together, these results demonstrated that BBR was able to protect RPE cells against oxidative stress via the activation of AMPK pathway. Our findings also indicate the potential application of BBR in AMD treatment.
KeywordAge-related macular degeneration AMPK Berberine D407 cells
DOI10.3390/ijms19061736
URLView the original
Language英語
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.UM Zhuhai Research Institute
2.Zhongshan Ophthalmic Center
3.Universidade de Macau
4.Southern Medical University
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GB/T 7714
Li S.,Gaur U.,Chong C.-M.,et al. Berberine protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of AMPK[J]. International Journal of Molecular Sciences,2018,19(6).
APA Li S..,Gaur U..,Chong C.-M..,Lin S..,Fang J..,...&Zheng W..(2018).Berberine protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of AMPK.International Journal of Molecular Sciences,19(6).
MLA Li S.,et al."Berberine protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of AMPK".International Journal of Molecular Sciences 19.6(2018).
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