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Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector
Liu, Fang1; Ma, Ni2; He, Chengwei1; Hu, Yuanjia1; Li, Peng1; Chen, Meiwan1; Su, Huanxing1; Wan, Jian-Bo1
2018-04
Source PublicationJOURNAL OF GINSENG RESEARCH
ISSN1226-8453
Volume42Issue:2Pages:149-157
Abstract

Background

Panax notoginseng leaves (PNL) exhibit extensive activities, but few analytical methods have been established to exclusively determine the dammarane triterpene saponins in PNL.

Methods

Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOF MS) and HPLC-UV methods were developed for the qualitative and quantitative analysis of ginsenosides in PNL, respectively.

Results

Extraction conditions, including solvents and extraction methods, were optimized, which showed that ginsenosides Rc and Rb3, the main components of PNL, are transformed to notoginsenosides Fe and Fd, respectively, in the presence of water, by removing a glucose residue from position C-3 via possible enzymatic hydrolysis. A total of 57 saponins were identified in the methanolic extract of PNL by UPLC/Q-TOF MS. Among them, 19 components were unambiguously characterized by their reference substances. Additionally, seven saponins of PNL—ginsenosides Rb1, Rc, Rb2, and Rb3, and notoginsenosides Fc, Fe, and Fd—were quantified using the HPLC-UV method after extraction with methanol. The separation of analytes, particularly the separation of notoginsenoside Fc and ginsenoside Rc, was achieved on a Zorbax ODS C8 column at a temperature of 35°C. This developed HPLC-UV method provides an adequate linearity (r2 > 0.999), repeatability (relative standard deviation, RSD < 2.98%), and inter- and intraday variations (RSD < 4.40%) with recovery (98.7–106.1%) of seven saponins concerned. This validated method was also conducted to determine seven components in 10 batches of PNL.

Conclusion

These findings are beneficial to the quality control of PNL and its relevant products.

KeywordGinsenoside Transformation Notoginsenoside Fd Notoginsenoside Fe Panax Notoginseng Leaves Uplc/q-tof Ms
DOI10.1016/j.jgr.2017.01.007
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine
WOS IDWOS:000428602500004
PublisherKOREAN SOC GINSENG
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWan, Jian-Bo
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Department of Product Development, Wenshan Sanqi Institute of Science and Technology, Wenshan University, Wenshan, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Liu, Fang,Ma, Ni,He, Chengwei,et al. Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector[J]. JOURNAL OF GINSENG RESEARCH,2018,42(2):149-157.
APA Liu, Fang.,Ma, Ni.,He, Chengwei.,Hu, Yuanjia.,Li, Peng.,...&Wan, Jian-Bo.(2018).Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector.JOURNAL OF GINSENG RESEARCH,42(2),149-157.
MLA Liu, Fang,et al."Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector".JOURNAL OF GINSENG RESEARCH 42.2(2018):149-157.
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